Estrogen exacerbates the nociceptive effects of peripheral serotonin on rat trigeminal sensory neurons
| Autor: | Taylor M. Hickman, Sirima Tongkhuya, Hanna Rose McDonald, Sukhbir Kaur, Sushmitha Ananth, Cierra M.C. Lopez, Dayna L. Averitt |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Serotonin
Orofacial pain medicine.medical_specialty 5-HT2A receptor Neuroscience (miscellaneous) TRPV1 Pain Neurosciences. Biological psychiatry. Neuropsychiatry Sensory system chemistry.chemical_compound Internal medicine medicine 5HT2A receptor Trigeminal sensory neurons 5-HT receptor Original Research business.industry Estrogen Anesthesiology and Pain Medicine Endocrinology Nociception chemistry Capsaicin Neurology (clinical) medicine.symptom business RC321-571 |
| Zdroj: | Neurobiology of Pain, Vol 10, Iss, Pp 100073-(2021) Neurobiology of Pain |
| ISSN: | 2452-073X |
| DOI: | 10.1016/j.ynpai.2021.100073 |
| Popis: | Highlights • 5HT-evoked nocifensive behaviors are sexually dimorphic and dependent on hormone status of the animal. • Blocking the excitatory 5HT2A receptor attenuates 5HT-evoked pain behaviors in females during proestrus/estrus. • Basal levels of 5HT are elevated in cycling females as compared to males. • Estrogen potentiates serotonergic neuromodulation of capsaicin-evoked CGRP release in female trigeminal sensory neurons. Orofacial pain disorders involving trigeminal sensory neurons disproportionately affect women and can be modulated by hormones, especially estrogen (E2). Proinflammatory mediators, like serotonin (5HT), can act on sensory neurons expressing the transient receptor potential vanilloid 1 (TRPV1) ion channel, resulting in peripheral sensitization. We previously reported peripheral 5HT evokes greater pain behaviors in the hindpaw of female rats during proestrus and estrus, stages when E2 fluctuates. It is unknown if this interaction is comparable in the trigeminal system. We hypothesized that E2 exacerbates 5HT-evoked nocifensive pain behaviors and pain signaling in female trigeminal sensory neurons. We report 5HT-evoked nocifensive behaviors are significantly higher during estrus and proestrus, which is attenuated by blocking the 5HT2A receptor. The comparable dose of 5HT was not nociceptive in males unless capsaicin was also administered. When administered with capsaicin, a lower dose of 5HT evoked trigeminal pain behaviors in females during proestrus. Further, basal 5HT content in the vibrissal pad was higher in cycling females compared to males. Ex vivo, E2 enhanced 5HT-potentiated CGRP release from trigeminal neurons, which was not significantly reduced by blocking the 5HT2A receptor. Our data indicates that estrogen fluctuation influences the pronociceptive effects of 5HT on trigeminal sensory neurons. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|