Binding of lurasidone, a novel antipsychotic, to rat 5-HT7 receptor: Analysis by [3H]SB-269970 autoradiography
| Autor: | Mutsuo Taiji, Tadashi Ishibashi, Michiko Ono, Takeo Ishiyama, Tomoko Horisawa |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Male
medicine.drug_class Atypical antipsychotic CHO Cells Isoindoles Pharmacology 5-HT7 receptor Rats Sprague-Dawley Lurasidone Hydrochloride Radioligand Assay SB-269970 Cricetinae medicine Radioligand Animals Receptor Biological Psychiatry 5-HT receptor Lurasidone Chemistry Brain Receptor antagonist Rats Thiazoles Receptors Serotonin Autoradiography Antipsychotic Agents medicine.drug |
| Zdroj: | Progress in Neuro-Psychopharmacology and Biological Psychiatry. 40:132-137 |
| ISSN: | 0278-5846 |
| Popis: | Lurasidone is a novel antipsychotic agent with high affinity for dopamine D(2) and serotonin 5-HT(7), 5-HT(2A), and 5-HT(1A) receptors. We previously reported that in addition to its antipsychotic action, lurasidone shows beneficial effects on mood and cognition in rats, likely through 5-HT(7) receptor antagonistic actions. In this study, we evaluated binding of lurasidone to 5-HT(7) receptors in the rat brain by autoradiography using [(3)H]SB-269970, a specific radioligand for 5-HT(7) receptors. Brain slices were incubated with 4 nM [(3)H]SB-269970 at room temperature and exposed to imaging plates for 8 weeks before phosphorimager analysis. Using this method, we first investigated 5-HT(7) receptor distribution. We found that 5-HT(7) receptors are abundantly localized in brain limbic structures, including the lateral septum, thalamus, hypothalamus, hippocampus, and amygdala. On the other hand, its distribution was moderate in the cortex and low in the caudate putamen and cerebellum. Secondly, binding of lurasidone, a selective 5-HT(7) receptor antagonist SB-656104-A and an atypical antipsychotic olanzapine to this receptor was examined. Lurasidone, SB-656104-A (10–1000 nM), and olanzapine (100–10,000 nM) showed concentration-dependent inhibition of [(3)H]SB-269970 binding with IC(50) values of 90, 49, and 5200 nM, respectively. Similar inhibitory actions of these drugs were shown in in vitro [(3)H]SB-269970 binding to 5-HT(7) receptors expressed in Chinese hamster ovary cells. Since there was no marked species difference in rat and human 5-HT(7) receptor binding by lurasidone (K(i) = 1.55 and 2.10 nM, respectively), these findings suggest that binding to 5-HT(7) receptors might play some role in its beneficial pharmacological actions in schizophrenic patients. |
| Databáze: | OpenAIRE |
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