Investigating Migraine-Like Behavior using Light Aversion in Mice
| Autor: | Levi P. Sowers, Andrew F. Russo, Mengya Wang, Adisa Kuburas, Bianca N. Mason, Brandon J. Rea |
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| Rok vydání: | 2021 |
| Předmět: |
Photophobia
Calcitonin Gene-Related Peptide Migraine Disorders General Chemical Engineering Neurological disorder Calcitonin gene-related peptide Optogenetics Motor Activity Open field Article General Biochemistry Genetics and Molecular Biology Mice medicine Animals Behavior Animal General Immunology and Microbiology business.industry General Neuroscience medicine.disease Migraine Brain stimulation Anxiety medicine.symptom business Neuroscience |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| DOI: | 10.3791/62839-v |
| Popis: | Migraine is a complex neurological disorder characterized by headache and sensory abnormalities, such as hypersensitivity to light, observed as photophobia. Whilst it is impossible to confirm that a mouse is experiencing migraine, light aversion can be used as a behavioral surrogate for the migraine symptom of photophobia. To test for light aversion, we utilize the light/dark assay to measure the time mice freely choose to spend in either a light or dark environment. The assay has been refined by introducing two critical modifications: pre-exposures to the chamber prior to running the test procedure and adjustable chamber lighting, permitting the use of a range of light intensities from 55 lux to 27,000 lux. Because the choice to spend more time in the dark is also indicative of anxiety, we also utilize a light-independent anxiety test, the open field assay, to distinguish anxiety from light-aversive behavior. Here, we describe a modified test paradigm for the light/dark and open field assays. The application of these assays is described for intraperitoneal injection of calcitonin gene-related peptide (CGRP) in two mouse strains and for optogenetic brain stimulation studies. |
| Databáze: | OpenAIRE |
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