Distribution of lasl and rhlR virulent gene among Staphylococcus aureus isolates in clinical samples from Ekiti state university teaching hospital, Ado Ekiti, Nigeria

Autor: Olawale David Ajayi, Onasanya, Amos Adeyinka, Ayodele Abiodun Ojo, Tajudeen Olabisi Obafemi, Daramola, Gabriel Olugbenga
Jazyk: angličtina
Rok vydání: 2023
Předmět:
DOI: 10.5281/zenodo.7919814
Popis: Infection withStaphylococcus aureushas been considered a major problem in hospitals. The clinical importance ofS. aureusis attributed to notable virulence factors and genetic diversity. The study aimed to investigate the distribution oflasIandrhlRvirulent gene (a quorum sensing system reported inPseudomonas aeruginosa)and different genotypes in some clinical isolates obtained from Ekiti State University Teaching Hospital, Ado Ekiti. A total of 100 clinical isolates were collected and screened out of which 75 samples with goodS. aureusyield were selected for this study. 34 (45.3%) were from males and 41 (54.7%) were from females. The isolates were drawn from across several age groups and sample types. Result showed that most susceptible antibiotics was Ciprofloxacin (64.0 %), while the most resistant antibiotics was Ampiclox (88.0%). Using Polymerase Chain Reaction (PCR), virulent geneslasLandrhiRformed clusters inS. aureusisolates used in this study,laslwas absence in 22 of the isolates and only present in 53 (71%) of the isolates,rhlRwas absent in 24 of the isolates and only present in 51 (68%) of the isolates. Virulence diversity was observed among isolates. FurthermoreS. aureuswas found to harborlasLandrhlR virulent gene,a quorum sensing system which was only reported inPseudomonas aeruginosa,revealing the virulent mutants in the isolates which could be used as a guide to the pathogenicity of individual isolates and hence control spread of infection. The genetic typing was carried out by Random Amplified Polymorphic DNA (RAPD-PCR) using OPH06, OPT06, OPT07 and OPHO7, primers. In conclusion there exist different genotypes which are mutants among theS. aureusisolates used in this study revealing high level of genetic diversity occurrence amongS. aureusisolates. The DNA fingerprint revealed high genetic diversity among isolates irrespective of their sources.
Databáze: OpenAIRE