The Saccharomyces cerevisiae peroxisomal 2,4-dienoyl-CoA reductase is encoded by the oleate-inducible gene SPS19
| Autor: | A. Gurvitz, Maximilian Binder, Barbara Hamilton, J K Hiltunen, Seppo H. Kilpeläinen, Ian W. Dawes, Hanspeter Rottensteiner, Andreas Hartig |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Fatty Acid Desaturases
Oxidoreductases Acting on CH-CH Group Donors Response element Saccharomyces cerevisiae Molecular Sequence Data 2 4 Dienoyl-CoA reductase Biology Reductase Biochemistry Microbodies Gene Expression Regulation Enzymologic Animals Humans Amino Acid Sequence Promoter Regions Genetic Molecular Biology Reporter gene Peroxisomal matrix Promoter Cell Biology Peroxisome biology.organism_classification Cell Compartmentation Rats Oxidation-Reduction Gene Deletion Oleic Acid |
| Zdroj: | The Journal of biological chemistry. 272(35) |
| ISSN: | 0021-9258 |
| Popis: | beta-Oxidation is compartmentalized in mammals into both mitochondria and peroxisomes. Fatty acids with double bonds at even-numbered positions require for their degradation the auxiliary enzyme 2,4-dienoyl-CoA reductase, and at least three isoforms, two mitochondrial and one peroxisomal, exist in the rat. The Saccharomyces cerevisiae Sps19p is 34% similar to the human and rat mitochondrial reductases, and an SPS19 deleted strain was unable to utilize petroselineate (cis-C18:1(6)) as the sole carbon source, but remained viable on oleate (cis-C18:1(9)). Sps19p was purified to homogeneity from oleate-induced cells and the homodimeric enzyme (native molecular weight 69,000) converted 2,4-hexadienoyl-CoA into 3-hexenoyl-CoA in an NADPH-dependent manner and therefore contained 2,4-dienoyl-CoA reductase activity. Antibodies raised against Sps19p decorated the peroxisomal matrix of oleate-induced cells. SPS19 shares with the sporulation-specific SPS18 a common promoter region that contains an oleate response element. This element unidirectionally regulates transcription of the reductase and is sufficient for oleate induction of a promoterless CYC1-lacZ reporter gene. SPS19 is dispensable for growth and sporulation on solid acetate and oleate media, but is essential for these processes to occur on petroselineate. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|