Transformation of Probiotic Yeast and Their Recovery from Gastrointestinal Immune Tissues Following Oral Gavage in Mice
| Autor: | Lauren E. Hudson, Taryn P. Stewart, Milo B. Fasken, Tracey J. Lamb, Anita H. Corbett |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
General Chemical Engineering Auxotrophy Immunology Administration Oral Heterologous Saccharomyces cerevisiae Biology Bioinformatics General Biochemistry Genetics and Molecular Biology law.invention Mice 03 medical and health sciences Probiotic Drug Delivery Systems Immune system Immunity law Animals Immunity Mucosal General Immunology and Microbiology Probiotics General Neuroscience biology.organism_classification Yeast Intestines Transformation (genetics) 030104 developmental biology Saccharomyces boulardii |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | Development of recombinant oral therapy would allow for more direct targeting of the mucosal immune system and improve the ability to combat gastrointestinal disorders. Adapting probiotic yeast in particular for this approach carries several advantages. These strains have not only the potential to synthesize a wide variety of complex heterologous proteins but are also capable of surviving and protecting those proteins during transit through the intestine. Critically, however, this approach requires expertise in many diverse laboratory techniques not typically used in tandem. Furthermore, although individual protocols for yeast transformation are well characterized for commonly used laboratory strains, emphasis is placed here on alternative approaches and the importance of optimizing transformation for less well characterized probiotic strains. Detailing these methods will help facilitate discussion as to the best approaches for testing probiotic yeast as oral drug delivery vehicles and indeed serve to advance the development of this novel strategy for gastrointestinal therapy. |
| Databáze: | OpenAIRE |
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