Selective Detection of Nano-Sized Diagnostic Markers Using Au-ZnO Nanorod-Based Surface-Enhanced Raman Spectroscopy (SERS) in Ureteral Obstruction Models
| Autor: | Sanghwa Lee, Jung-Man Namgoong, Myung-Soo Choo, Yujin Joung, Miyeon Jue, Chae-Min Ryu, Dong-Myung Shin, Jun Ki Kim |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
principal component analysis
Pharmaceutical Science 02 engineering and technology Urine Kidney Spectrum Analysis Raman urologic and male genital diseases 01 natural sciences ureteral obstruction Rats Sprague-Dawley International Journal of Nanomedicine Drug Discovery Original Research Nanotubes nano-sized biomarker Chemistry General Medicine 021001 nanoscience & nanotechnology surface-enhanced Raman spectroscopy medicine.anatomical_structure symbols Female Kidney Diseases Ureteral Stricture Nanorod Collagen Zinc Oxide 0210 nano-technology renal injury Phenylalanine Biophysics ZnO nanorods Bioengineering Urinalysis 010402 general chemistry Biomaterials symbols.namesake Renal injury medicine Animals urogenital system Organic Chemistry technology industry and agriculture Diagnostic marker Surface-enhanced Raman spectroscopy Fibrosis 0104 chemical sciences Disease Models Animal Gold Raman spectroscopy Biomarkers Biomedical engineering |
| Zdroj: | International Journal of Nanomedicine |
| ISSN: | 1178-2013 |
| Popis: | Sanghwa Lee,1,* Jung-Man Namgoong,2,* Miyeon Jue,1 Yujin Joung,1 Chae-Min Ryu,3,4 Dong-Myung Shin,4 Myung-Soo Choo,3 Jun Ki Kim1,5 1Biomedical Engineering Research Center, Asan Medical Center, Seoul 05505, Republic of Korea; 2Department of Surgery, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 3Department of Urology, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 4Department of Biomedical Sciences, Asan Medical Center, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea; 5Department of Convergence Medicine, University of Ulsan College of Medicine, Seoul 05505, Republic of Korea*These authors contributed equally to this workCorrespondence: Jun Ki Kim Email kim@amc.seoul.krBackground: This study investigated the diagnosis of renal diseases using a biochip capable of detecting nano-sized biomarkers. Raman measurements from a kidney injury model were taken, and the feasibility of early diagnosis was assessed.Materials and Methods: Rat models with mild and severe unilateral ureteral obstructions were created, with the injury to the kidney varying according to the tightness of the stricture. After generating the animal ureteral obstruction models, urine was collected from the kidney and bladder.Results and Discussion: After confirming the presence of renal injury, urine drops were placed onto a Raman chip whose surface had been enhanced with Au-ZnO nanorods, allowing nano-sized biomarkers that diffused into the nanogaps to be selectively amplified. The Raman signals varied according to the severity of the renal damage, and these differences were statistically confirmed.Conclusion: These results confirm that ureteral stricture causes kidney injury and that signals in the urine from the release of nano-biomarkers can be monitored using surface-enhanced Raman spectroscopy.Keywords: ureteral obstruction, renal injury, nano-sized biomarker, ZnO nanorods, surface-enhanced Raman spectroscopy, principal component analysis |
| Databáze: | OpenAIRE |
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