Identification of [CuCl(acac)(tmed)], a copper(II) complex with mixed ligands, as a modulator of Cu,Zn superoxide dismutase (Sod1p) activity in yeast
| Autor: | Ileana C. Farcasanu, Ion Baciu, Augustin M. Ofiteru, Cristian D. Ene, Codruta C. Paraschivescu, Augustin M. Madalan, Ioana Dumitru |
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| Rok vydání: | 2012 |
| Předmět: |
Saccharomyces cerevisiae Proteins
Stereochemistry Saccharomyces cerevisiae chemistry.chemical_element medicine.disease_cause Ligands Biochemistry Scavenger Inorganic Chemistry Superoxide dismutase chemistry.chemical_compound Structure-Activity Relationship Coordination Complexes medicine chemistry.chemical_classification biology Dose-Response Relationship Drug Molecular Structure Chemistry Superoxide biology.organism_classification Copper Yeast Enzyme Activation Enzyme biology.protein Oxidative stress Molecular Chaperones |
| Zdroj: | Journal of biological inorganic chemistry : JBIC : a publication of the Society of Biological Inorganic Chemistry. 17(6) |
| ISSN: | 1432-1327 |
| Popis: | Superoxide dismutases (SODs) stand in the prime line of enzymatic antioxidant defense in nearly all eukaryotic cells exposed to oxygen, catalyzing the breakdown of the superoxide anionic radical to O(2) and H(2)O(2). Overproduction of superoxide correlates with numerous pathophysiological conditions, and although the native enzyme can be used as a therapeutic agent in superoxide-associated conditions, synthetic low molecular weight mimetics are preferred in terms of cost, administration mode, and bioavailability. In this study we make use of the model eukaryote Saccharomyces cerevisiae to investigate the SOD-mimetic action of a mononuclear mixed-ligand copper(II) complex, [CuCl(acac)(tmed)] (where acac is acetylacetonate anion and tmed is N,N,N',N'-tetramethylethylenediamine). Taking advantage of an easily reproducible phenotype of yeast cells which lack Cu-Zn SOD (Sod1p), we found that the compound could act either as a superoxide scavenger in the absence of native Sod1p or as a Sod1p modulator which behaved differently under various genetic backgrounds. |
| Databáze: | OpenAIRE |
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