No evidence for bradykinin B1 receptors in rat dorsal root ganglion neurons
| Autor: | M Petersen, Monika Brand, A Klusch, Miguel Valdeolmillos, Robert Franz Schmidt, Oliver Kurzai |
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| Rok vydání: | 2001 |
| Předmět: |
Agonist
Intracellular Fluid Male medicine.medical_specialty Fura-2 medicine.drug_class Receptor expression Tachyphylaxis Bradykinin Receptor Bradykinin B1 Drug Administration Schedule Rats Sprague-Dawley chemistry.chemical_compound Calcium imaging Dorsal root ganglion Internal medicine Ganglia Spinal medicine Animals Calcium Signaling Neurons Afferent RNA Messenger Bradykinin receptor Rats Wistar Cells Cultured Fluorescent Dyes General Neuroscience Receptors Bradykinin Peripheral Nervous System Diseases Anatomy Kallidin Blotting Northern Rats Endocrinology medicine.anatomical_structure chemistry Hyperalgesia Neuralgia Calcium medicine.symptom |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 0959-4965 |
| Popis: | Bradykinin receptors are believed to contribute to hyperalgesia under conditions of neuropathic pain. Using calcium imaging we investigated responses to B1 and B2 agonists on isolated rat dorsal root ganglion neurons. No response to the B1 agonist was detected, whereas 12% of neurons responded to the B2 agonist. Northern blot analysis confirmed the lack of B1 receptor expression in dorsal root ganglia, as B1 mRNA was neither detected under normal conditions nor after nerve injury. In the calcium imaging experiments, agonists were applied with an elevated superfusion flow rate to avoid tachyphylaxis to the drug. Normal external solution applied at this flow rate constituted a mechanical stimulus causing a response in some neurons. Thus, in comparable set-ups mechanosensitivity has first to be tested to avoid masking effects. |
| Databáze: | OpenAIRE |
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