Macroporous hydrogels derived from aqueous dynamic phase separation
| Autor: | Marcy Zenobi-Wong, Srinivas Madduri, Frank Bradke, Andreas Husch, Nicolas Broguiere, Gemma Palazzolo |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
Biomedical
02 engineering and technology Hippocampus Polyethylene Glycols Extracellular matrix chemistry.chemical_compound Engineering Biomimetics Ganglia Spinal Hyaluronic Acid Materials drug effects [Nerve Regeneration] metabolism [Nerve Net] Cells Cultured chemistry [Cross-Linking Reagents] Neurons 0303 health sciences pharmacology [Hyaluronic Acid] Chemistry chemistry [Water] Hydrogels Adhesion 021001 nanoscience & nanotechnology Sciatic Nerve Porous Step-growth polymerization chemistry [Polyethylene Glycols] Cross-Linking Reagents metabolism [Neurons] Mechanics of Materials Self-healing hydrogels Macroporous Hydrogel Neuron Tissue Microstructures Bioengineering Neural Networks Nerve 0210 nano-technology Porosity Biocompatibility Neurite Biophysics Polyethylene glycol chemistry [Hydrogels] physiology [Sciatic Nerve] Phase Transition Biomaterials 03 medical and health sciences Polysaccharides ddc:570 drug effects [Ganglia Spinal] drug effects [Nerve Net] PEG ratio drug effects [Neurons] Animals 030304 developmental biology chemistry [Polysaccharides] drug effects [Sciatic Nerve] Water Nerve Regeneration Rats Kinetics cytology [Hippocampus] Ceramics and Composites Nerve Net metabolism [Ganglia Spinal] |
| Zdroj: | Biomaterials, 200 Biomaterials 200, 56-65 (2019). doi:10.1016/j.biomaterials.2019.01.047 |
| ISSN: | 1878-5905 |
| DOI: | 10.1016/j.biomaterials.2019.01.047 |
| Popis: | A method to generate injectable macroporous hydrogels based on partitioning of polyethylene glycol (PEG) and high viscous polysaccharides is presented. Step growth polymerization of PEG was used to initiate a phase separation and the formation of a connected macroporous network with tunable dimensions. The possibilities and physical properties of this new category of materials were examined, and then applied to address some challenges in neural engineering. First, non-degradable macroporous gels were shown to support rapid neurite extension from encapsulated dorsal root ganglia (DRGs) with unprecedented long-term stability. Then, dissociated primary rat cortical neurons could be encapsulated with >95% viability, and extended neurites at the fast rate of ≈100 μm/day and formed synapses, resulting in functional, highly viable and long-term stable 3D neural networks in the synthetic extracellular matrix (ECM). Adhesion cues were found unnecessary provided the gels have optimal physical properties. Normal electrophysiological properties were confirmed on 3D cultured mouse hippocampal neurons. Finally, the macroporous gels supported axonal growth in a rat sciatic nerve injury model when used as a conduit filling. The combination of injectability, tunable pore size, stability, connectivity, transparency, cytocompatibility and biocompatibility, makes this new class of materials attractive for a wide range of applications. |
| Databáze: | OpenAIRE |
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