Genetic diversity and population structure of the endangered marsupial Sarcophilus harrisii (Tasmanian devil)
| Autor: | Stephan C. Schuster, Qingyu Wang, Gregory M. Woods, Desiree C. Petersen, James W. Knight, Jason R. Miller, Oscar C. Bedoya-Reina, Arthur M. Lesk, Stephen Pyecroft, Neerja Katiyar, Yu Zhang, Menna E. Jones, Fangqing Zhao, Nicola E. Wittekindt, Paula Woodbridge, Elizabeth A. Tindall, Lynn P. Tomsho, Dale Dixon, Ji Qi, Rae-Anne Hardie, Mads F. Bertelsen, Vanessa M. Hayes, Brian P. Walenz, Kristofer M. Helgen, Thomas H. Pringle, Lindsay Mc Clellan Kasson, Webb Miller, Nick Patterson, Aakrosh Ratan, Alexandre Kreiss |
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| Rok vydání: | 2011 |
| Předmět: |
Models
Molecular Time Factors Population Molecular Sequence Data Devil facial tumour disease Population genetics Breeding Extinction Biological DNA Mitochondrial Polymorphism Single Nucleotide Tasmania Neoplasms Tasmanian devil Genetic variation medicine Animals Humans education Phylogeny Marsupial Genetics education.field_of_study Genetic diversity Multidisciplinary biology Genetic Variation DNA Neoplasm Biological Sciences biology.organism_classification medicine.disease Neoplasm Proteins Sarcophilus Genetics Population Marsupialia Evolutionary biology Genome Mitochondrial Facial Neoplasms |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 108(30) |
| ISSN: | 1091-6490 |
| Popis: | The Tasmanian devil ( Sarcophilus harrisii ) is threatened with extinction because of a contagious cancer known as Devil Facial Tumor Disease. The inability to mount an immune response and to reject these tumors might be caused by a lack of genetic diversity within a dwindling population. Here we report a whole-genome analysis of two animals originating from extreme northwest and southeast Tasmania, the maximal geographic spread, together with the genome from a tumor taken from one of them. A 3.3-Gb de novo assembly of the sequence data from two complementary next-generation sequencing platforms was used to identify 1 million polymorphic genomic positions, roughly one-quarter of the number observed between two genetically distant human genomes. Analysis of 14 complete mitochondrial genomes from current and museum specimens, as well as mitochondrial and nuclear SNP markers in 175 animals, suggests that the observed low genetic diversity in today's population preceded the Devil Facial Tumor Disease disease outbreak by at least 100 y. Using a genetically characterized breeding stock based on the genome sequence will enable preservation of the extant genetic diversity in future Tasmanian devil populations. |
| Databáze: | OpenAIRE |
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