An alginate/poly(N-isopropylacrylamide)-based composite hydrogel dressing with stepwise delivery of drug and growth factor for wound repair
| Autor: | Xilin Lin, Lin Du, Mei Tu, Jianhao Zhao, Xipeng Guan, Youheng Wu, Jianhua Rong, Yuzheng Wu, Shuqiang Zhuang, Jiafeng Zhao |
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| Rok vydání: | 2020 |
| Předmět: |
Male
Materials science Diclofenac Angiogenesis Alginates medicine.medical_treatment Basic fibroblast growth factor Acrylic Resins Anti-Inflammatory Agents Nanogels Bioengineering Human skin 02 engineering and technology 010402 general chemistry 01 natural sciences Biomaterials chemistry.chemical_compound In vivo medicine Animals Humans Wound Healing Growth factor Diclofenac Sodium 021001 nanoscience & nanotechnology Bandages 0104 chemical sciences Rats Disease Models Animal chemistry Mechanics of Materials Poly(N-isopropylacrylamide) Angiogenesis Inducing Agents Fibroblast Growth Factor 2 0210 nano-technology Wound healing Biomedical engineering |
| Zdroj: | Materials scienceengineering. C, Materials for biological applications. 115 |
| ISSN: | 1873-0191 |
| Popis: | Anti-inflammation and angiogenesis play an essential role in wound healing. In this study, we developed a composite hydrogel dressing with stepwise delivery of diclofenac sodium (DS) and basic fibroblast growth factor (bFGF) in the inflammation stage and new tissue formation stage respectively for wound repair. Sodium alginate (SA) crosslinked by calcium ion acted as the continuous phase, and thermosensitive bFGF-loaded poly(N-isopropylacrylamide) nanogels (pNIPAM NGs, LCST1 ~33 °C) and DS-loaded p(N-isopropylacrylamide-co-acrylic acid) nanogels [p(NIPAM-co-AA) NGs, LCST2 ~40 °C] acted as the dispersed phase. The synthesized SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) hydrogel presented a desirable storage modulus of ~4500 Pa, a high water equilibrium swelling ratio of ~90, an appropriate water vapor transmission rate of ~2300 g/m2/day, and nontoxicity to human skin fibroblasts. The in vitro thermosensitive cargo delivery of this hydrogel showed that 92% of DS was sustainably delivered at 37 °C within the early three days mimicking the inflammation stage, while 80% of bFGF was controlled released at 25 °C within the later eight days mimicking new tissue formation stage. The in vivo wound healing of rats showed that this composite hydrogel presented a better healing effect with a wound contraction of 96% at 14 d, less inflammation and higher angiogenesis, than all control groups. These findings indicate SA/bFGF@pNIPAM/DS@p(NIPAM-co-AA) composite hydrogel is a potential dressing for wound repair. |
| Databáze: | OpenAIRE |
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