Immunosorbent assays for diagnosis of Crimean-Congo hemorrhagic fever (CCHF)
| Autor: | E. A. Tkachenko, Donets Ma, Rezapkin Gv, Ivanov Ap |
|---|---|
| Rok vydání: | 1982 |
| Předmět: |
Crimean–Congo hemorrhagic fever
Hemagglutination Guinea Pigs Radioimmunoassay Enzyme-Linked Immunosorbent Assay Immunofluorescence Antibodies Viral Immunoglobulin G Mice Antigen Virology medicine Animals Antigens Viral medicine.diagnostic_test biology biology.organism_classification medicine.disease Rats Infectious Diseases Hemorrhagic Fever Virus Crimean-Congo biology.protein Immunologic Techniques Parasitology Hemorrhagic Fever Crimean Antibody Bunyaviridae |
| Zdroj: | The American journal of tropical medicine and hygiene. 31(1) |
| ISSN: | 0002-9637 |
| Popis: | Utilization of direct and indirect enzyme-linked immunosorbent assay (ELISA) and solid-phase radioimmunoassay (SPRIA) for the diagnosis of Crimean-Congo hemorrhagic fever (CCHF) allows the detection of low amounts of infectious virus (2 log LD50) or inactivated antigen and antibody to CCHF within 5-6 hours. These methods were shown to be more sensitive, specific, rapid, and reproducible than the complement-fixation test, immunofluorescence, hemagglutination, or radial diffusion in gel. The experimental design of ELISA and SPRIA developed for CCHF may be used successfully for the detection of the other members of the Bunyaviridae family. |
| Databáze: | OpenAIRE |
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