Microfibrils at Basement Membrane Zones Interact with Perlecan via Fibrillin-1
| Autor: | Rupert Timpl, Holger Notbohm, Kerstin Tiedemann, Erika Gustafsson, Ursula Schlötzer-Schrehardt, Dieter P. Reinhardt, Takako Sasaki, Thilo Wedel, B. Bätge, Walter Göhring |
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| Rok vydání: | 2005 |
| Předmět: |
Fibrillin-1
Perlecan Fibrillins Biochemistry Basement Membrane Extracellular matrix Mice Fibrillin Microfibrils medicine Animals Humans Fluorescent Antibody Technique Indirect Molecular Biology Skin Basement membrane biology Chemistry Microfilament Proteins Colocalization Cell Biology carbohydrates (lipids) medicine.anatomical_structure Proteoglycan Microfibrils biology.protein Biophysics Heparitin Sulfate Fibrillin Heparan Sulfate Proteoglycans |
| Zdroj: | Journal of Biological Chemistry. 280:11404-11412 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m409882200 |
| Popis: | Mutational defects in fibrillin-rich microfibrils give rise to a number of heritable connective tissue disorders, generally termed microfibrillopathies. To understand the pathogenesis of these microfibrillopathies, it is important to elucidate the supramolecular composition of microfibrils and their interaction properties with extracellular matrix components. Here we demonstrate that the proteoglycan perlecan is an associated component of microfibrils typically close to basement membrane zones. Double immunofluorescence studies demonstrate colocalization of fibrillin-1, the major backbone component of microfibrils, with perlecan in fibroblast cultures as well as in dermal and ocular tissues. Double immunogold labeling further confirms colocalization of perlecan to microfibrils in various tissues at the ultrastructural level. Extraction studies revealed that perlecan is not covalently associated with microfibrils. High affinity interactions between fibrillin-1 and perlecan were found by kinetic binding studies with dissociation constants in the low nanomolar range. A detailed mapping study of the interaction epitopes by solid phase binding assays primarily revealed interactions of perlecan domains I and II with a central region of fibrillin-1. Analysis of perlecan null embryos showed less microfibrils at the dermal-epidermal junction as compared with wild-type littermates. The data presented indicate a functional significance for perlecan in anchoring microfibrils to basement membranes and in the biogenesis of microfibrils. |
| Databáze: | OpenAIRE |
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