Coronary Revascularization During Heart Regeneration Is Regulated by Epicardial and Endocardial Cues and Forms a Scaffold for Cardiomyocyte Repopulation

Autor: Sofia-Iris Bibli, Sri Teja Mullapuli, Rubén Marín-Juez, Kenneth D. Poss, Ingrid Fleming, Aosa Kamezaki, Christian S. M. Helker, Didier Y.R. Stainier, Hadil El-Sammak, Matthew J. Foglia
Rok vydání: 2019
Předmět:
Receptors
CXCR4
medicine.medical_specialty
Heart Ventricles
medicine.medical_treatment
Neovascularization
Physiologic
Infarction
Biology
Revascularization
Article
General Biochemistry
Genetics and Molecular Biology
03 medical and health sciences
0302 clinical medicine
Internal medicine
Myocardial Revascularization
medicine
Animals
Regeneration
Myocytes
Cardiac
cardiovascular diseases
Myocardial infarction
Molecular Biology
Zebrafish
Endocardium
Cell Proliferation
030304 developmental biology
Wound Healing
0303 health sciences
Heart
Cell Biology
Zebrafish Proteins
Hypoxia (medical)
medicine.disease
biology.organism_classification
Chemokine CXCL12
Apelin
Vascular endothelial growth factor A
cardiovascular system
Cardiology
Cues
medicine.symptom
Pericardium
030217 neurology & neurosurgery
Signal Transduction
Developmental Biology
Zdroj: Dev Cell
ISSN: 1534-5807
DOI: 10.1016/j.devcel.2019.10.019
Popis: Defective coronary network function and insufficient blood supply are both cause and consequence of myocardial infarction. Efficient revascularization after infarction is essential to support tissue repair and function. Zebrafish hearts exhibit a remarkable ability to regenerate, and coronary revascularization initiates within hours of injury, but how this process is regulated remains unknown. Here we show that revascularization requires a coordinated multi-tissue response culminating with the formation of a complex vascular network available as a scaffold for cardiomyocyte repopulation. During a process we term “coronary-endocardial anchoring”, new coronaries respond by sprouting 1) superficially within the regenerating epicardium, and 2) intra-ventricularly towards the activated endocardium. Mechanistically, superficial revascularization is guided by epicardial Cxcl12-Cxcr4 signaling, and intra-ventricular sprouting by endocardial Vegfa signaling. Our findings indicate that the injury-activated epicardium and endocardium support cardiomyocyte replenishment initially through the guidance of coronary sprouting. Simulating this process in the injured mammalian heart should help its healing.
Databáze: OpenAIRE
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