Differential evolution of cell-associated virus in blood and genital tract of HIV-infected females undergoing HAART
| Autor: | Grissell Tirado, Gladys Sepulveda, Dhirendra Pratap Singh, Yasuhiro Yamamura, Evelyn Reyes, Anil Kumar, Gloria Jove |
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| Jazyk: | angličtina |
| Předmět: |
Adult
HAART medicine.medical_treatment viruses Cell Molecular Sequence Data HIV Infections Drug resistance Biology Peripheral blood mononuclear cell Virus Evolution Molecular 03 medical and health sciences 0302 clinical medicine HIV Protease Antiretroviral Therapy Highly Active Virology Drug Resistance Viral medicine Humans 030212 general & internal medicine Amino Acid Sequence Cell-associated virus Gene Polymerase Gene Phylogeny 030304 developmental biology 0303 health sciences Protease HIV Middle Aged Genes pol Reverse transcriptase HIV Reverse Transcriptase 3. Good health Phylogenetics medicine.anatomical_structure Mutation Vagina Virus compartmentalization HIV-1 Leukocytes Mononuclear Female |
| Zdroj: | Virology. (2):299-305 |
| ISSN: | 0042-6822 |
| DOI: | 10.1016/j.virol.2005.01.030 |
| Popis: | PBMC and vaginal cell (VC) viruses were studied from 5 HIV-infected females for the presence of drug-resistance and non-drug resistance associated mutations. A 1318-bp fragment of polymerase gene was amplified from PBMC and VC proviral DNA. Four of the 5 PBMC viruses exhibited drug resistance-associated mutations in reverse transcriptase and protease genes, whereas only 2 VC viruses contained drug resistance-associated mutations. However, all 5 females showed non-drug resistance-associated mutations both in PBMC and VC virus suggesting continuous evolution of the virus in these compartments. The emergence of drug resistance was slower in PBMC and VC viruses than that observed in the cell-free plasma (P) and vaginal secretion (VS) viruses. Phylogenetic analysis revealed that VC virus was closer to PBMC virus than either cell-free viruses (P and VS) suggesting comparable evolution among cell-associated viruses. |
| Databáze: | OpenAIRE |
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