The Effect of Single and Combined Use of Gamma Radiation and Ethylmethane Sulfonate on Early Growth Parameters in Sorghum
| Autor: | Hussein Shimelis, Maliata Athon Wanga, Fatma Sarsu, Lydia N. Horn |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Veterinary medicine Combined use Plant Science 01 natural sciences Article 03 medical and health sciences Genetic variation Ethylmethane Sulfonate Ecology Evolution Behavior and Systematics Mutation breeding Ecology biology gamma radiation Botany food and beverages ethylmethane sulfonate Sorghum biology.organism_classification 030104 developmental biology Seedling QK1-989 sorghum Dose rate 010606 plant biology & botany |
| Zdroj: | Plants Volume 9 Issue 7 Plants, Vol 9, Iss 827, p 827 (2020) |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants9070827 |
| Popis: | Success in inducing genetic variation through mutagenic agents is dependent on the source and dose of application. The objective of this study was to determine the optimum doses of a single and combined use of gamma radiation and ethylmethane sulfonate (EMS) for effective mutation breeding in sorghum. The study involved two concurrent experiments as follows: in experiment I, the seeds of four sorghum genotypes (&lsquo Parbhani Moti&rsquo &lsquo Parbhani Shakti&rsquo ICSV 15013&prime and &lsquo Macia&rsquo ) were treated using gamma radiation (0, 300, 400, 500 and 600 Gy), EMS (0, 0.5 and 1.0%), and gamma radiation followed by EMS (0 and 300 Gy and 0.1% EMS 400 Gy and 0.05% EMS). In experiment II, the seeds of two genotypes (&lsquo Red sorghum&rsquo ) were treated with seven doses of gamma radiation only (0, 100, 200, 300, 400, 500 and 600 Gy). Overall, the combined applied doses of gamma radiation and EMS are not recommended due to poor seedling emergence and seedling survival rate below LD50. The best dosage of gamma radiation for genotypes Red sorghum, Parbhani Moti, Macia, ICSV 15013 and Parbhani Shakti ranged between 392 and 419 Gy, 311 and 354 Gy, 256 and 355 Gy, 273 and 304 Gy, and 266 and 297 Gy, respectively. The EMS optimum dosage ranges for genotypes Parbhani Shakti, ICSV 15013, Parbhani Moti and Macia were between 0.41% and 0.60%, 0.48% and 0.58%, 0.46% and 0.51%, and 0.36% and 0.45%, respectively. The above dose rates are useful to induce genetic variation in the tested sorghum genotypes for greater mutation events in sorghum breeding programs. |
| Databáze: | OpenAIRE |
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