Transcriptome dataset of Babesia bovis life stages within vertebrate and invertebrate hosts
| Autor: | Kelly A. Brayton, Wendell C. Johnson, Kathryn E. Reif, Joana C. Silva, Michelle R. Mousel, Olukemi O. Ifeonu, Carlos E. Suarez, David R. Herndon, Massaro W. Ueti, Lowell S. Kappmeyer, Naomi S. Taus |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Transovarial transmission
Babesia Tick lcsh:Computer applications to medicine. Medical informatics Transcriptome 03 medical and health sciences 0302 clinical medicine Kinetes parasitic diseases Parasite hosting lcsh:Science (General) Gene 030304 developmental biology Data Article Genetics 0303 health sciences Multidisciplinary biology Babesia bovis Bovine biology.organism_classification Rhipicephalus microplus lcsh:R858-859.7 Gene expression Bovine babesiosis 030217 neurology & neurosurgery lcsh:Q1-390 |
| Zdroj: | Data in Brief Data in Brief, Vol 33, Iss, Pp 106533-(2020) |
| ISSN: | 2352-3409 |
| Popis: | Babesia bovis is a hemoprotozoan parasite of cattle that has a complex life cycle within vertebrate and invertebrate hosts. In the mammalian host, B. bovis undergoes asexual reproduction while in the tick midgut, gametes are induced, fuse, and form zygotes. The zygote infects tick gut epithelial cells and transform into kinetes that are released into the hemolymph and invade other tick tissues such as the ovaries, resulting in transovarial transmission to tick offspring. To compare gene regulation between different B. bovis life stages, we collected parasites infecting bovine erythrocytes and tick hemolymph. Total RNA samples were isolated, and multiplexed libraries sequenced using paired-end 100 cycle reads of a HiSeq 2500. The data was normalized using the TMM method and analysed for significant differential expression using the generalized linear model likelihood ratio test (GLM LRT) in edgeR. To validate our datasets, ten genes were selected using NormFinder. Genes that had no significant fold change between the blood and tick stages in the RNA-Seq datasets were tested by quantitative PCR to determine their suitability as "housekeeping" genes. The normalized RNA-Seq data revealed genes upregulated during infection of the mammalian host or tick vector and six upregulated genes were validated by quantitative PCR. These datasets can help identify useful targets for controlling bovine babesiosis. |
| Databáze: | OpenAIRE |
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