Antimalarial artesunate protects sepsis model mice against heat-killed Escherichia coli challenge by decreasing TLR4, TLR9 mRNA expressions and transcription factor NF-κB activation
| Autor: | Guofu Ding, Sheng-Qi He, Weiwei Jiang, Yan Dong, Lezhi Zhang, Jiang Zheng, Hongwei Cao, Rong Zhang, Ping Luo, Bin Li, Jun Li, Yongling Lu, Hong Zhou |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Male
Lipopolysaccharide CpG Oligodeoxynucleotide medicine.medical_treatment Immunology Artesunate Bacteremia Biology Proinflammatory cytokine Antimalarials Mice chemistry.chemical_compound medicine Animals Immunology and Allergy RNA Messenger Cells Cultured Escherichia coli Infections Pharmacology Interleukin-6 Tumor Necrosis Factor-alpha NF-kappa B TLR9 Molecular biology Artemisinins Endotoxins Toll-Like Receptor 4 Cytokine Oligodeoxyribonucleotides chemistry Toll-Like Receptor 9 TLR4 Female Human umbilical vein endothelial cell Tumor necrosis factor alpha Sesquiterpenes |
| Zdroj: | International Immunopharmacology. 8:379-389 |
| ISSN: | 1567-5769 |
| DOI: | 10.1016/j.intimp.2007.10.024 |
| Popis: | Bacterial DNA (bDNA) and lipopolysaccharide (LPS) are potent activators of immune cells such as monocytes and macrophages, which contribute to systemic inflammatory response syndrome (SIRS) and sepsis. Unfortunately, many experimental inflammatory antagonist-based therapies have failed in sepsis trials, and currently there is only one adjuvant therapy in clinical use, e.g. activated protein C. Artesunate (AS), a water-soluble derivative of dihydroartemisinin, has recently been demonstrated to protect against LPS-induced human umbilical vein endothelial cell (HUVEC) activation and injury by inhibiting tumor necrosis factor-alpha (TNF-alpha) mRNA expression. In the present study, heat-killed Escherichia coli was used to induce sepsis in the animal models. We observed that AS could protect mice against a lethal challenge with heat-killed E. coli in a dose-dependent manner. This protection was associated with reductions in serum TNF-alpha and measurable endotoxin levels. In addition, the treatment of murine peritoneal macrophage cells with AS strongly inhibited the release of TNF-alpha and IL-6 induced by CpG oligodeoxynucleotide (CpG ODN), LPS, or heat-killed E. coli in a dose-dependent manner. Experiments using affinity sensor technology revealed that AS could not directly bind to CpG ODN or LPS. Moreover, AS could not neutralize LPS in vitro. Further, flow cytometry revealed that AS could not alter the binding of CpG ODN to cell surfaces but could promote CpG ODN accumulation within RAW264.7 cells. Furthermore, AS reduced the expressions of TLR4 and TLR9 mRNA that were stimulated by LPS, CpG ODN, or heat-killed E. coli and inhibited heat killed E. coli-induced NF-kappaB activation. In conclusion, our results demonstrated that AS-mediated protection against a lethal heat-killed E. coli challenge was associated with a reduction in proinflammatory cytokine release and endotoxin levels via a mechanism involving a decrease in TLR4, TLR9 mRNA expression and NF-kappaB activation. |
| Databáze: | OpenAIRE |
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