Danhong injection facilitates recovery of post-stroke motion deficit via Parkin-enhanced mitochondrial function
| Autor: | Xiumei Gao, John Orgah, Xinyan Liu, Jie Ren, Yan Zhu, Emmanuel A. Orgah |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
030506 rehabilitation
Ubiquitin-Protein Ligases Mitochondrion Pharmacology Neuroprotection Parkin Brain Ischemia Random Allocation 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Paralysis medicine Animals Rats Wistar Survival rate Cells Cultured Gait Disorders Neurologic Neurons business.industry Therapeutic effect Recovery of Function Minocycline humanities Mitochondria Rats Stroke Disease Models Animal Neuroprotective Agents Neurology Immunohistochemistry Neurology (clinical) medicine.symptom 0305 other medical science business 030217 neurology & neurosurgery Drugs Chinese Herbal medicine.drug |
| Zdroj: | Restorative Neurology and Neuroscience. 37:375-395 |
| ISSN: | 1878-3627 0922-6028 |
| DOI: | 10.3233/rnn-180828 |
| Popis: | Background A cerebral ischemic stroke involves mitochondrial dysfunction, motor deficits, and paralysis; and Danhong injection (DHI) might possess mitochondrial protection and functional recovery in a stroke subject through promoting expression of parkin, a ubiquitin ligase playing a key role in the regulation of proteins and mitochondria quality control. Objective To investigate the therapeutic effects of DHI on the histological, cellular, and functional recovery of Wistar rats after middle cerebral artery occlusion/reperfusion (MCAO/R). Methods One hundred and twenty healthy male Wistar rats (250-300 g), were randomly assigned to six groups (twenty rats/group). Rats were subjected to 1 h MCAO/R and subsequently administered the intravenous doses of DHI (0.75, 1.5, and 3 mL/kg) to the respective groups (twice a day for 14 days). Unlike the other groups, the sham group received surgery without vessel occlusion. All the animals were tested for gait behavior using the CatWalk system. The body weight/survival rates were recorded daily for 14 days. The parkin protein expression of the brain tissue was quantified by immunohistochemistry analysis. Additionally, cultured cortical neurons were incubation with DHI or minocycline (MC) and then deprived of oxygen and glucose for 2 h (to resemble ischemic/reperfusion), followed by 4 h reoxygenation. Cellular and mitochondrial phenotypes were assayed by high content analysis. Results Neurological integrity and paw parameters of the animals were altered in the model group but significantly ameliorated by DHI administration. Also, the infarct volume and survival rate were significantly improved in DHI groups. DHI enhanced the expression of parkin protein in the brain and improved the relative mitochondrial reductase activity of the cultured neurons. Conclusions The overall result shows that daily intervention with DHI provides neuroprotection and survival to improve gait motion in Wistar rats. |
| Databáze: | OpenAIRE |
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