The nuclear localization of WAP and CSN genes is modified by lactogenic hormones in HC11 cells

Autor: Pierre Adenot, Cathy Hue-Beauvais, Eve Devinoy, Kiên Kiêu, Maria Ballester, Clémence Kress, Gaetan Lehmann
Přispěvatelé: Unité de recherche génomique et physiologie de la lactation (GPL), Institut National de la Recherche Agronomique (INRA), Unité de biométrie et intelligence artificielle de jouy, Biologie du développement et reproduction (BDR), Centre National de la Recherche Scientifique (CNRS)-École nationale vétérinaire d'Alfort (ENVA)-Institut National de la Recherche Agronomique (INRA)
Rok vydání: 2008
Předmět:
Zdroj: Journal of Cellular Biochemistry
Journal of Cellular Biochemistry, Wiley, 2008, 105 (1), pp.262-270. ⟨10.1002/jcb.21823⟩
ISSN: 0730-2312
1097-4644
DOI: 10.1002/jcb.21823
Popis: International audience; Whey acidic protein (WAP) and casein (CSN) genes are among the most highly expressed milk protein genes in the mammary gland of the lactating mouse. Their tissue-specific regulation depends on the activation and recruitment of transcription factors, and chromatin modifications in response to hormonal stimulation. We have investigated if another mechanism, such as specific positioning of the genes in the nucleus, could be involved in their functional regulation. Fluorescent in situ hybridization was used to study the nuclear localization of WAP and CSN genes in mouse mammary epithelial cells (HC11) cultured in the absence and presence of lactogenic hormones. Automatic 3D image processing and analysis tools were developed to score gene positions. In the absence of lactogenic hormones, both genes are distributed non-uniformly within the nucleus: the CSN locus was located close to the nuclear periphery and the WAP gene tended to be central. Stimulation by lactogenic hormones induced a statistically significant change to their distance from the periphery, which has been described as a repressive compartment. The detection of genes in combination with the corresponding chromosome-specific probe revealed that the CSN locus is relocated outside its chromosome territory following hormonal stimulation, whereas the WAP gene, which is already sited more frequently outside its chromosome territory in the absence of hormones, is not affected. We conclude that milk protein genes are subject to nuclear repositioning when activated, in agreement with a role for nuclear architecture in gene regulation, but that they behave differently as a function of their chromosomal context.
Databáze: OpenAIRE
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