The varicella-zoster virus induces apoptosis in vitro in subpopulations of primary human peripheral blood mononuclear cells
| Autor: | Andreas König, Claudia Hömme, Manfred H Wolff, Alexandra Dietrich, Bärbel Hauröder |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Herpesvirus 3
Human viruses Lymphocyte Immunology Apoptosis Biology medicine.disease_cause Microbiology Peripheral blood mononuclear cell Virus Herpesviridae Alphaherpesvirinae medicine In Situ Nick-End Labeling Humans Annexin A5 Cells Cultured Cell Nucleus integumentary system Cell Membrane Varicella zoster virus virus diseases DNA biology.organism_classification Virology Molecular biology Microscopy Electron Infectious Diseases medicine.anatomical_structure Leukocytes Mononuclear DNA fragmentation Benzimidazoles Propidium |
| Zdroj: | Microbes and infection. 5(10) |
| ISSN: | 1286-4579 |
| Popis: | Varicella-zoster virus (VZV), a member of Herpesviridae, subfamily alpha-Herpesvirinae, is pathogenic exclusively in the human. Chickenpox is the result of primary infection of VZV. During the viremic stage, VZV infects peripheral blood mononuclear cells (PBMC) and spreads to the periphery. In skin cells it causes typical lesions. Apoptosis has been demonstrated in different cell types by other alpha-herpesviruses. VZV-infected T lymphocytes, B lymphocytes, and monocytes, respectively, were examined in this in vitro study by flow cytometry, immunofluorescence and electron microscopy. All infected cell types showed signs of apoptosis: a lower DNA content, DNA fragmentation, loss of membrane integrity, and an altered nuclear morphology. The results observed led to the suggestion that VZV can induce apoptosis during infection in vivo in the PBMC subpopulations. |
| Databáze: | OpenAIRE |
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