Development of an In Vitro Model for the Disruption of Extracellular Matrix Components in the Study of Congenital Renal Parenchymal Dysmorphism
| Autor: | Nancy Weber Kaye, Steven C. Howe, William J. Cromie |
|---|---|
| Rok vydání: | 1989 |
| Předmět: |
Pathology
medicine.medical_specialty Urology Mesenchyme Abnormalities Drug-Induced Cell Differentiation Chick Embryo Anatomy In Vitro Techniques Biology Kidney In vitro Extracellular Matrix In vitro model Extracellular matrix Kidney Tubules medicine.anatomical_structure Tubule Norleucine Parenchyma medicine Animals Humans Microinjection Histological examination |
| Zdroj: | Journal of Urology. 142:612-615 |
| ISSN: | 1527-3792 0022-5347 |
| DOI: | 10.1016/s0022-5347(17)38832-8 |
| Popis: | We attempted to refine a model system for the study of renal parenchymal malformations and demonstrate its use with a preliminary study of the induction of congenital renal dysmorphism. A technique is described whereby 3-day chick embryos are explanted from their shells and cultured in vitro in specially constructed polyethylene chambers. At 8 days of development, the time of ureteral induction of the metanephric mesenchyme, 6-diazo-5-oxo-norleucine, an inhibitor of proteoglycan biosynthesis, is administered as a single microinjection into the posterolateral body wall. Histological examination of fixed metanephric tissue revealed a gross deficiency in the number of differentiated tubules in norleucine-treated animals. A morphometric analysis was made of the actual area occupied by differentiated tubules in the norleucine-treated and control kidneys between 10 and 15 days of development. This analysis revealed a total tubule surface area of control kidneys of more than 2 times greater than that of embryos injected with norleucine at ureteral induction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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