Surfactant-free production of biomimetic giant unilamellar vesicles using PDMS-based microfluidics
| Autor: | Julien Petit, Tom Robinson, Oliver Bäumchen, Naresh Yandrapalli |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Membrane lipids
Microfluidics 02 engineering and technology 010402 general chemistry 01 natural sciences Biochemistry law.invention chemistry.chemical_compound law Materials Chemistry Glycerol Environmental Chemistry QD1-999 Liposome Artificial cell Vesicle General Chemistry Lab-on-a-chip 021001 nanoscience & nanotechnology 0104 chemical sciences Chemistry Membrane chemistry Biophysics lipids (amino acids peptides and proteins) 0210 nano-technology |
| Zdroj: | Communications Chemistry bioRxiv Communications Chemistry, Vol 4, Iss 1, Pp 1-10 (2021) |
| Popis: | Microfluidic production of giant lipid vesicles presents a paradigm-shift in the development of artificial cells. While production is high-throughput and the lipid vesicles are mono-disperse compared to bulk methods, current technologies rely heavily on the addition of additives such as surfactants, glycerol and even ethanol. Here we present a microfluidic method for producing biomimetic surfactant-free and additive-free giant unilamellar vesicles. The versatile design allows for the production of vesicle sizes ranging anywhere from ~10 to 130 µm with either neutral or charged lipids, and in physiological buffer conditions. Purity, functionality, and stability of the membranes are validated by lipid diffusion, protein incorporation, and leakage assays. Usability as artificial cells is demonstrated by increasing their complexity, i.e., by encapsulating plasmids, smaller liposomes, mammalian cells, and microspheres. This robust method capable of creating truly biomimetic artificial cells in high-throughput will prove valuable for bottom-up synthetic biology and the understanding of membrane function. The production of giant unilamellar vesicles by microfluidics commonly involves additives, which may interfere with the resultant membrane properties. Here pure lipid GUVs are prepared which exclude residual surfactants and other additives. |
| Databáze: | OpenAIRE |
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