Tumor Protein p53 and K-ras Gene Mutations in Peruvian Patients with Gallbladder Cancer
| Autor: | Jessica I. Aramburu, Kazutoshi Nakamura, Ebert Poquioma, Ayako Sato, Takao Asai, Monica Calderon, Tatiana Vidaurre, Sandro Casavilca, Paola Catherine Montenegro, Toshikazu Ikoma, Jeannie Navarro, Yoichi Ajioka, Henry L. Gomez, Yasuo Tsuchiya |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Silent mutation Adult Male Bolivia Gene mutation medicine.disease_cause Proto-Oncogene Mas Proto-Oncogene Proteins p21(ras) 03 medical and health sciences 0302 clinical medicine Peru Medicine Missense mutation Humans Gallbladder cancer tumor suppressor gene Aged Aged 80 and over Mutation business.industry Gallbladder Point mutation proto-oncogene General Medicine Exons Middle Aged medicine.disease 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Cancer research Female Gallbladder Neoplasms Gallbladder Neoplasm Tumor Suppressor Protein p53 business Research Article |
| Zdroj: | Asian Pacific Journal of Cancer Prevention : APJCP |
| ISSN: | 2476-762X |
| Popis: | Background: Recent studies have shown that genetic alterations are associated with the effect of patient geographic location on gallbladder cancer development. Peru has a high incidence of gallbladder cancer, but causative factors have not yet been identified. We examined the frequency of mutations in TP53 and K -ras genes in Peruvian patients with gallbladder cancer, and compared this with data from Bolivia, Hungary, Chile, and Japan, which have a high gallbladder cancer incidence. Methods: DNA was extracted from formalin-fixed paraffin-embedded gallbladder tissue sections of 30 gallbladder cancer patients (9 men and 21 women) obtained using microdissection. Mutations in exons 5 to 8 of TP53 and codons 12, 13, and 61 of K -ras were examined using direct sequencing. Results: TP53 mutations were observed in 10 (33.3%) of patients, but K-ras mutations were absent. Nine (90%) TP53 mutations were point mutations (7 missense and 2 silent mutations), and the most frequent substitution was a G:C to A:T transition. G:C to A:T transitions at the CpG site or G:C to T:A transversions were found in one patient each. No significant differences were found in the frequency of TP53 and K-ras mutations among patients in the 5 countries. Conclusions: Our findings suggest that endogenous mechanisms and exogenous carcinogens may affect the carcinogenic process in Peruvian gallbladder cancer patients, similar to that in Bolivian patients. Further studies with a larger sample size are needed to clarify these findings. |
| Databáze: | OpenAIRE |
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