A single dose polyanhydride-based nanovaccine against paratuberculosis infection
Autor: | Kathleen A. Ross, Adel M. Talaat, Balaji Narasimhan, Yashdeep Phanse, Howard Steinberg, Chungyi Hansen, Akanksha Thukral |
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Jazyk: | angličtina |
Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
lcsh:Immunologic diseases. Allergy medicine.medical_treatment T cell Immunology Paratuberculosis 02 engineering and technology lcsh:RC254-282 Article Microbiology 03 medical and health sciences Immunity medicine Pharmacology (medical) Adjuvants Pathogen Pharmacology Vaccines business.industry Intracellular parasite 021001 nanoscience & nanotechnology medicine.disease lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens 030104 developmental biology Infectious Diseases Cytokine medicine.anatomical_structure Inactivated vaccine 0210 nano-technology business lcsh:RC581-607 CD8 |
Zdroj: | npj Vaccines, Vol 5, Iss 1, Pp 1-10 (2020) NPJ Vaccines |
ISSN: | 2059-0105 |
Popis: | Mycobacterium avium subsp. paratuberculosis (M. paratuberculosis) causes Johne’s disease in ruminants and is characterized by chronic gastroenteritis leading to heavy economic losses to the dairy industry worldwide. The currently available vaccine (inactivated bacterin in oil base) is not effective in preventing pathogen shedding and is rarely used to control Johne’s disease in dairy herds. To develop a better vaccine that can prevent the spread of Johne’s disease, we utilized polyanhydride nanoparticles (PAN) to encapsulate mycobacterial antigens composed of whole cell lysate (PAN-Lysate) and culture filtrate (PAN-Cf) of M. paratuberculosis. These nanoparticle-based vaccines (i.e., nanovaccines) were well tolerated in mice causing no inflammatory lesions at the site of injection. Immunological assays demonstrated a substantial increase in the levels of antigen-specific T cell responses post-vaccination in the PAN-Cf vaccinated group as indicated by high percentages of triple cytokine (IFN-γ, IL-2, TNF-α) producing CD8+ T cells. Following challenge, animals vaccinated with PAN-Cf continued to produce significant levels of double (IFN-γ, TNF-α) and single cytokine (IFN-γ) secreting CD8+ T cells compared with animals vaccinated with an inactivated vaccine. A significant reduction in bacterial load was observed in multiple organs of animals vaccinated with PAN-Cf, which is a clear indication of protection. Overall, the use of polyanhydride nanovaccines resulted in development of protective and sustained immunity against Johne’s disease, an approach that could be applied to counter other intracellular pathogens. |
Databáze: | OpenAIRE |
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