The T-box transcription factor Eomesodermin acts upstream of Mesp1 to specify cardiac mesoderm during mouse gastrulation
| Autor: | Elizabeth J. Robertson, Sebastian J. Arnold, Inga-Marie Pimeisl, Ita Costello, Elizabeth K. Bikoff, Sarah Dräger |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Transcriptional Activation Mesoderm Mice 129 Strain Nodal Protein Mesp1/2 Blotting Western Green Fluorescent Proteins Molecular Sequence Data Eomesodermin Mammalian embryology Mice Transgenic Biology Article Mice 03 medical and health sciences Cell Line Tumor Sequence Homology Nucleic Acid Basic Helix-Loop-Helix Transcription Factors medicine Animals gastrulation cardiac specification In Situ Hybridization 030304 developmental biology 0303 health sciences Base Sequence Reverse Transcriptase Polymerase Chain Reaction Primitive streak Myocardium 030302 biochemistry & molecular biology Cell Biology Embryo Mammalian Cell biology Mice Inbred C57BL Gastrulation medicine.anatomical_structure Microscopy Fluorescence embryonic structures Female definitive endoderm T-Box Domain Proteins NODAL Transcription Factor Gene Definitive endoderm |
| Zdroj: | Nature cell biology |
| Popis: | Instructive programmes guiding cell-fate decisions in the developing mouse embryo are controlled by a few so-termed master regulators. Genetic studies demonstrate that the T-box transcription factor Eomesodermin (Eomes) is essential for epithelial-to-mesenchymal transition, mesoderm migration and specification of definitive endoderm during gastrulation. Here we report that Eomes expression within the primitive streak marks the earliest cardiac mesoderm and promotes formation of cardiovascular progenitors by directly activating the bHLH (basic-helix-loop-helix) transcription factor gene Mesp1 upstream of the core cardiac transcriptional machinery. In marked contrast to Eomes/Nodal signalling interactions that cooperatively regulate anterior-posterior axis patterning and allocation of the definitive endoderm cell lineage, formation of cardiac progenitors requires only low levels of Nodal activity accomplished through a Foxh1/Smad4-independent mechanism. Collectively, our experiments demonstrate that Eomes governs discrete context-dependent transcriptional programmes that sequentially specify cardiac and definitive endoderm progenitors during gastrulation. |
| Databáze: | OpenAIRE |
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