Laboratory identification and enteropathogenicity testing of Serpulina pilosicoli associated with porcine colonic spirochetosis
| Autor: | Michelle R. Mathiesen, Nagaraja Muniappa, Gerald E. Duhamel |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
0301 basic medicine
Serpulina hyodysenteriae Brachyspira Time Factors Genotype 040301 veterinary sciences Swine Serpulina innocens Spirochaetales Infections Polymerase Chain Reaction law.invention Microbiology 0403 veterinary science Caecum 03 medical and health sciences Cecum Colonic Diseases law RNA Ribosomal 16S medicine Animals Large intestine Intestinal Mucosa Polymerase chain reaction Swine Diseases General Veterinary biology Virulence Hippurates 04 agricultural and veterinary sciences Ribosomal RNA biology.organism_classification 16S ribosomal RNA 030104 developmental biology medicine.anatomical_structure Phenotype Chickens |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 9(2) |
| ISSN: | 1040-6387 |
| Popis: | Pathogenic intestinal spirochetes of swine include Serpulina hyodysenteriae, a strongly s-hemolytic spirochete that causes swine dysentery, and S. pilosicoli, a weakly s-hemolytic intestinal spirochete (WBHIS) that causes porcine colonic spirochetosis. Because of the existence of nonpathogenic WBHIS in the normal swine colon, it is important to develop laboratory procedures for accurate identification of S. pilosicoli. The purpose of the present study was to assess hippurate hydrolysis and polymerase chain reaction (PCR) ampli- fication of specific 16S ribosomal RNA (rRNA) sequences for identification of porcine S. pilosicoli. Additionally, the enteropathogenicity of 8 field isolates of porcine S. pilosicoli was determined by challenge exposure of 1-day- old chicks and sequential histologic examination of the cecal mucosa. The field isolates of porcine S. pilosicoli hydrolyzed hippurate and yielded S. pilosicoli-specific products by PCR amplification of 16S rRNA sequences. Although all of the field isolates of porcine S. pilosicoli attached to the cecal epithelium of challenge-exp osed chicks by day 21 postinoculation, some isolates had locally invasive phenotypes. We concluded that identification of porcine S. pilosicoli could be made on the basis of results of hippurate hydrolysis and 16S rRNA PCR amplification. Challenge inoculation of 1-day-old chicks followed by histologic examination of the cecal mucosa demonstrated the enteropathogenicity of porcine S. pilosicoli. Previously, all weakly s-hemolytic intestinal spiro- Europe, and Australia. Additionally, S. pilosicoli has chetes (WBHIS) inhabiting the large intestine of swine a broad host range; spirochetes with a similar mor- were assigned to the nonpathogenic spirochete species, phology have been seen in the cecum and colon of Serpulina innocens. 1,13,16,17,28,29 The porcine WBHIS now human beings, 5,6,30 nonhuman primate, 5,30 dogs, 6 guin- have been shown to consist of a diverse group in ad- ea pigs, 22 and opossums 36 often with clinical signs or |
| Databáze: | OpenAIRE |
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