Primitive neural stem cells from the mammalian epiblast differentiate to definitive neural stem cells under the control of Notch signaling
| Autor: | Cheryl Koscik, Shoji Tsuji, Susumu Kusunoki, Derek van der Kooy, Raewyn M. Seaberg, Tania O. Alexson, Seiji Hitoshi, Ichiro Kanazawa |
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| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Embryoid body
Biology Leukemia Inhibitory Factor Research Communications Mice Neurosphere Genetics Animals Neurons Induced stem cells Receptors Notch Interleukin-6 Stem Cells Membrane Proteins Cell Differentiation Embryo Mammalian Embryonic stem cell Neural stem cell Mice Mutant Strains Cell biology Neuroepithelial cell Blastocyst Retroviridae Fibroblast Growth Factor 2 Stem cell Developmental Biology Adult stem cell Signal Transduction |
| Popis: | Basic fibroblast growth factor (FGF2)-responsive definitive neural stem cells first appear in embryonic day 8.5 (E8.5) mouse embryos, but not in earlier embryos, although neural tissue exists at E7.5. Here, we demonstrate that leukemia inhibitory factor-dependent (but not FGF2-dependent) sphere-forming cells are present in the earlier (E5.5–E7.5) mouse embryo. The resultant clonal sphere cells possess self-renewal capacity and neural multipotentiality, cardinal features of the neural stem cell. However, they also retain some nonneural properties, suggesting that they are the in vivo cells' equivalent of the primitive neural stem cells that form in vitro from embryonic stem cells. The generation of the in vivo primitive neural stem cell was independent of Notch signaling, but the activation of the Notch pathway was important for the transition from the primitive to full definitive neural stem cell properties and for the maintenance of the definitive neural stem cell state. |
| Databáze: | OpenAIRE |
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