Late Inflammation Induced by Asbestiform Fibers in Mice Is Ameliorated by a Small Molecule Synthetic Lignan
| Autor: | Kinta M. Serve, Melpo Christofidou-Solomidou, Reagan Badger, Ralph A. Pietrofesa, Kyewon Park |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Male
T-Lymphocytes medicine.medical_treatment Adaptive Immunity Pharmacology medicine.disease_cause Autoimmunity Mice Glucosides Biology (General) Butylene Glycols Chemokine CCL2 Spectroscopy B-Lymphocytes biology Asbestos Amphibole autoimmunity General Medicine Free radical scavenger Computer Science Applications Immunoglobulin Isotypes Chemistry Cytokine Female LGM2605 Antibody medicine.symptom QH301-705.5 Immunoglobulins asbestiform fibers Inflammation Article Catalysis Libby amphibole secoisolariciresinol diglucoside Inorganic Chemistry Immune system medicine Animals Physical and Theoretical Chemistry QD1-999 Molecular Biology Interleukin-6 business.industry Organic Chemistry asbestos Immunity Innate Mice Inbred C57BL Oxidative Stress Immunoglobulin class switching inflammation biology.protein business Oxidative stress |
| Zdroj: | International Journal of Molecular Sciences Volume 22 Issue 20 International Journal of Molecular Sciences, Vol 22, Iss 10982, p 10982 (2021) |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms222010982 |
| Popis: | Exposure to Libby amphibole (LA) asbestos-like fibers is associated with increased risk of asbestosis, mesothelioma, pulmonary disease, and systemic autoimmune disease. LGM2605 is a small molecule antioxidant and free radical scavenger, with anti-inflammatory effects in various disease models. The current study aimed to determine whether the protective effects of LGM2605 persist during the late inflammatory phase post-LA exposure. Male and female C57BL/6 mice were administered daily LGM2605 (100 mg/kg) via gel cups for 3 days before and 14 days after a 200 µg LA given via intraperitoneal (i.p.) injection. Control mice were given unsupplemented gel cups and an equivalent dose of i.p. saline. On day 14 post-LA treatment, peritoneal lavage was assessed for immune cell influx, cytokine concentrations, oxidative stress biomarkers, and immunoglobulins. During the late inflammatory phase post-LA exposure, we noted an alteration in trafficking of both innate and adaptive immune cells, increased pro-inflammatory cytokine concentrations, induction of immunoglobulin isotype switching, and increased oxidized guanine species. LGM2605 countered these changes similarly among male and female mice, ameliorating late inflammation and altering immune responses in late post-LA exposure. These data support possible efficacy of LGM2605 in the prolonged treatment of LA-associated disease and other inflammatory conditions. |
| Databáze: | OpenAIRE |
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