Transcriptomic analysis of salt stress responsive genes in Rhazya stricta
| Autor: | Ahmed M. Ramadan, Ahmed Atef, Neil Hall, Jamal S. M. Sabir, Fotouh M. El-Domyati, Madgy A. Al-Kordy, Robert K. Jansen, Abdullah Y. Obaid, Mohammed Z. Mutwakil, Nour O. Gadalla, Mohammad A. Khiyami, Rania M. Makki, Alawia Alhebshi, Charllotte A. Nelson, Dhivya Arasappan, Sherif Edris, Ahmed Bahieldin, Nahid H. Hajrah |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Salinity Leaves Chloroplasts Gene Expression lcsh:Medicine Plant Science 01 natural sciences Biochemistry Transcriptome Database and Informatics Methods Gene Expression Regulation Plant Plant Resistance to Abiotic Stress Cluster Analysis Enzyme Chemistry lcsh:Science Energy-Producing Organelles Regulation of gene expression Multidisciplinary biology Ecology Plant Anatomy High-Throughput Nucleotide Sequencing Salt Tolerance Mitochondria Chloroplast Multigene Family Plant Physiology Cellular Structures and Organelles Cellular Types Sequence Analysis Research Article Bioinformatics Plant Cell Biology Bioenergetics Rhazya stricta Research and Analysis Methods Enzyme Regulation 03 medical and health sciences Stress Physiological Plant-Environment Interactions Plant Cells Genetics Gene family Plant Defenses Gene Regulation Gene Gene Expression Profiling Plant Ecology Ecology and Environmental Sciences lcsh:R Computational Biology Biology and Life Sciences Proteins Cell Biology Plant Pathology biology.organism_classification Chaperone Proteins Apocynaceae Plant Leaves Metabolic pathway 030104 developmental biology Gene Ontology Tetrapyrroles Enzymology Pentatricopeptide repeat lcsh:Q Sequence Alignment 010606 plant biology & botany |
| Zdroj: | PLoS ONE, Vol 12, Iss 5, p e0177589 (2017) PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Rhazya stricta is an evergreen shrub that is widely distributed across Western and South Asia, and like many other members of the Apocynaceae produces monoterpene indole alkaloids that have anti-cancer properties. This species is adapted to very harsh desert conditions making it an excellent system for studying tolerance to high temperatures and salinity. RNA-Seq analysis was performed on R. stricta exposed to severe salt stress (500 mM NaCl) across four time intervals (0, 2, 12 and 24 h) to examine mechanisms of salt tolerance. A large number of transcripts including genes encoding tetrapyrroles and pentatricopeptide repeat (PPR) proteins were regulated only after 12 h of stress of seedlings grown in controlled greenhouse conditions. Mechanisms of salt tolerance in R. stricta may involve the upregulation of genes encoding chaperone protein Dnaj6, UDP-glucosyl transferase 85a2, protein transparent testa 12 and respiratory burst oxidase homolog protein b. Many of the highly-expressed genes act on protecting protein folding during salt stress and the production of flavonoids, key secondary metabolites in stress tolerance. Other regulated genes encode enzymes in the porphyrin and chlorophyll metabolic pathway with important roles during plant growth, photosynthesis, hormone signaling and abiotic responses. Heme biosynthesis in R. stricta leaves might add to the level of salt stress tolerance by maintaining appropriate levels of photosynthesis and normal plant growth as well as by the participation in reactive oxygen species (ROS) production under stress. We speculate that the high expression levels of PPR genes may be dependent on expression levels of their targeted editing genes. Although the results of PPR gene family indicated regulation of a large number of transcripts under salt stress, PPR actions were independent of the salt stress because their RNA editing patterns were unchanged. |
| Databáze: | OpenAIRE |
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