The long and winding road of reverse genetics in Trypanosoma cruzi
| Autor: | Noelia Lander, Miguel A. Chiurillo |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
Chagas disease
QH301-705.5 Computational biology Trypanosoma brucei Biochemistry Genetics and Molecular Biology (miscellaneous) Microbiology Applied Microbiology and Biotechnology gene knockout Genome editing Virology parasitic diseases Genetics medicine CRISPR genome editing Biology (General) Trypanosoma cruzi gene tagging Molecular Biology CRISPR/Cas9 biology Cas9 Cell Biology biology.organism_classification medicine.disease Leishmania Genetically modified organism Editorial gene knock-in Parasitology trypanosomes |
| Zdroj: | Microbial Cell Microbial Cell, Vol 8, Iss 9, Pp 203-207 (2021) |
| ISSN: | 2311-2638 |
| Popis: | Trypanosomes are early divergent protists with distinctive features among eukaryotic cells. Together with Trypanosoma brucei and Leishmania spp., Trypanosoma cruzi has been one of the most studied members of the group. This protozoan parasite is the causative agent of Chagas disease, a leading cause of heart disease in the Americas, for which there is no vaccine or satisfactory treatment available. Understanding T. cruzi biology is crucial to identify alternative targets for antiparasitic interventions. Genetic manipulation of T. cruzi has been historically challenging. However, the emergence of CRISPR/Cas9 technology has significantly improved the ability to generate genetically modified T. cruzi cell lines. Still, the system alone is not sufficient to answer all biologically relevant questions. In general, current genetic methods have limitations that should be overcome to advance in the study of this peculiar parasite. In this brief historic overview, we highlight the strengths and weaknesses of the molecular strategies that have been developed to genetically modify T. cruzi, emphasizing the future directions of the field. |
| Databáze: | OpenAIRE |
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