Excitable Adult-Generated GABAergic Neurons Acquire Functional Innervation in the Cortex after Stroke
| Autor: | Jagroop Dhaliwal, Yingben Xue, Jean-Claude Béïque, Diane C. Lagace, Anthony Carter, Timal S. Kannangara |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Doublecortin Domain Proteins
0301 basic medicine Aging Time Factors medicine.medical_treatment Biochemistry Brain Ischemia Nestin GABA Mice 0302 clinical medicine photothrombosis Cortex (anatomy) GABAergic Neurons Stroke lcsh:QH301-705.5 Cerebral Cortex education.field_of_study lcsh:R5-920 Neurogenesis subventricular zone stroke neurogenesis cortex Phenotype medicine.anatomical_structure GABAergic Stroke recovery lcsh:Medicine (General) Microtubule-Associated Proteins injury Green Fluorescent Proteins Population Subventricular zone ischemia Biology 03 medical and health sciences stem cells Report Precursor cell Genetics medicine Animals Cell Lineage cardiovascular diseases education brain repair Neuropeptides Cell Biology medicine.disease 030104 developmental biology lcsh:Biology (General) Synapses Neuroscience Biomarkers 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Stem Cell Reports, Vol 11, Iss 6, Pp 1327-1336 (2018) Stem Cell Reports |
| ISSN: | 2213-6711 |
| Popis: | Summary Ischemic stroke enhances the proliferation of adult-generated precursor cells that ectopically migrate toward the infarct. Studies have correlated precursor cell proliferation and subsequent adult neurogenesis with enhanced stroke recovery, yet it remains unclear whether stroke can generate new neurons capable of functional integration into the injured cortex. Here, using single and bitransgenic reporter mice, we identify spatial and temporal features of a multilineage cellular response to focal ischemia. We reveal that a small population of stroke-induced immature neurons accumulate within the peri-infarct region of the adult sensorimotor cortex, exhibit voltage-dependent conductances, fire action potentials, express GABAergic markers, and receive sparse GABAergic synaptic inputs. Collectively, these findings reveal that GABAergic neurons arising from the lateral ventricle have the capacity to integrate into the stroke-injured cortex, although their limited number and exiguous synaptic integration may limit their ability to participate in stroke recovery. Highlights • Stroke evokes a spatial and temporal precursor cell response in the cortex • DCX+ immature neurons accumulate in low numbers in the peri-infarct cortex • Immature neurons express markers for GABAergic neurons • Neurons exhibit action potentials and receive sparse GABAergic synaptic input In this article, Kannangara and colleagues histologically and electrophysiologically characterized the precursor cell response evoked in the adult cortex after focal ischemia. The authors identify a population of doublecortin-expressing GABAergic immature neurons localized to the injured cortical regions with the capacity to fire action potentials and receive GABAergic input, indicative of functionally integration into the cortical network. |
| Databáze: | OpenAIRE |
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