Screen Printing Tissue Models Using Chemically Cross-Linked Hydrogel Systems: A Simple Approach To Efficiently Make Highly Tunable Matrices
| Autor: | Michael A. LaScola, Yanchun Tang, Erin B. Lavik, Narendra Pandala, Maria Bieberich, LaShanda T. J. Korley |
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| Rok vydání: | 2021 |
| Předmět: |
Scaffold
food.ingredient Materials science Biomedical Engineering 3D printing Nanotechnology 02 engineering and technology Gelatin Biomaterials 03 medical and health sciences chemistry.chemical_compound food Tissue engineering Animals 030304 developmental biology 0303 health sciences Tissue Engineering Tissue Scaffolds business.industry Bioprinting Hydrogels 021001 nanoscience & nanotechnology Characterization (materials science) chemistry Printing Three-Dimensional Self-healing hydrogels Screen printing 0210 nano-technology business Ethylene glycol |
| Zdroj: | ACS Biomaterials Science & Engineering. 7:5007-5013 |
| ISSN: | 2373-9878 |
| DOI: | 10.1021/acsbiomaterials.1c00902 |
| Popis: | In vitro models provide a good starting point for drug screening and understanding various cellular mechanisms corresponding to different conditions. 3D cultures have drawn significant interest to mimic the in vivo microenvironment better and overcome the limitations of the 2D monolayered cultures. We previously reported a technique based on the screen printing process to pattern live mammalian cells using gelatin as the bioink. Even though gelatin is an inexpensive scaffolding material with various tissue engineering applications, it might not be the ideal hydrogel material to provide various mechanical and chemical cues to the cells. In this paper, we discuss the synthesis and characterization of two synthetic chemically cross-linked hydrogel systems based on poly(ethylene glycol) (PEG) and poly-l-lysine (PLL) to be used as the bioink in the screen printing process. These hydrogels are suitable as the bioinks for the screen printing process and serve as the barebone materials that can be tuned mechanically and augmented chemically to create a suitable in vitro microenvironment for the cells. This paper presents the synthesis, mechanical testing, and characterization of the hydrogel systems and their applications in the screen printing process. |
| Databáze: | OpenAIRE |
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