Blood group ABO antigen expression in human embryonic stem cells and in differentiated hepatocyte- and cardiomyocyte-like cells
Autor: | Michael E. Breimer, Johan Mölne, Anders Jeppsson, Petter Björquist, Katarina Andersson, Valeri Strokan, Mette Diswall, Lennart Rydberg |
---|---|
Rok vydání: | 2008 |
Předmět: |
Genotype
Cell Culture Techniques Polymerase Chain Reaction ABO Blood-Group System Cell therapy Immune system Antigen ABO blood group system Humans Myocytes Cardiac Progenitor cell Embryonic Stem Cells Polymorphism Single-Stranded Conformational Transplantation biology Cell Differentiation Embryonic stem cell Molecular biology Blood Group Incompatibility Immunology biology.protein Blood Group Antigens Hepatocytes Tissue and Organ Harvesting Chromatography Thin Layer Antibody Stem cell Glycolipids |
Zdroj: | Transplantation. 86(10) |
ISSN: | 1534-6080 |
Popis: | Background. The use of stem cells in regenerative medicine and transplantation may require grafting of cells that will challenge the recipient’s immune system. Our knowledge of tissue antigen expression in human embryonic stem cells (hESC) and during their differentiation is limited, especially regarding histo-blood group AB(O)H antigens. Methods. Nine different hESC lines, and hESC-derived hepatocyte- and cardiomyocyte-like cells, were blood group ABO genotyped and A/B antigen expression was studied by immunohistochemistry. Results. This study reveals, for the first time, that A and B antigens in hESC were expressed according to the ABO genotype and that the antigens had a different cellular/sub-cellular distribution. In addition, several genotype A hESC lines stained positive with one anti-B antibody. Furthermore, studies of hepatocyte- and cardiomyocyte-like cells of different maturation state, originating from a blood group B hESC line, showed that hepatocyte-like cells expressed B antigens whereas cardiomyocyte-like cells were negative. Conclusion. Since clinical stem-cell therapy is likely to be performed with immature progenitor cells, blood group ABO compatibility of donor cells/recipients should be favorable to avoid unnecessary rejection problems caused by ABO incompatibility. The in vitro loss of B antigens in a genotype B hESC line indicates that loss of ABH antigens occurs early during human embryogenesis since these antigens are lacking in adult cardiomyocytes. |
Databáze: | OpenAIRE |
Externí odkaz: |