Quality and fertility of frozen ovine spermatozoa from epididymides stored at room temperature (18-25 °C) for up to 48 h post mortem

Autor: Tácia Gomes Bergstein-Galan, Eduarda Maciel Busato, Sony Dimas Bicudo, Ana Claudia Machinski Rangel de Abreu, Luiz Ernandes Kozicki, Melina Andrea Formighieri Bertol, Romildo Romualdo Weiss
Přispěvatelé: Federal University of Paraná, Pontifical Catholic University of Paraná, Universidade Estadual Paulista (Unesp)
Rok vydání: 2017
Předmět:
Zdroj: Scopus
Repositório Institucional da UNESP
Universidade Estadual Paulista (UNESP)
instacron:UNESP
ISSN: 1879-3231
Popis: Made available in DSpace on 2018-12-11T17:11:02Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-07-01 This study investigates the effect of time of storage of epididymides at room temperature and the addition of 20% of seminal plasma to the cryopreservation extender, on post thaw quality and fertility of ovine spermatozoa collected from the cauda epididymis. Spermatic kinetics, integrity and the stability of plasma membrane, damage to the acrosome and fertility following laparoscopic artificial insemination were evaluated in samples collected in an artificial vagina (AV) and from epididymides stored at room temperature for zero (G0), six (G6), twelve (G12), twenty-four (G24) and forty-eight (G48) hours post mortem. There were no significant differences in spermatic parameters between the methods of sample collection, except for progressive motility and velocity according to the straight path(VSL). G48 samples had significant lower total motility(TM), progressive motility(PM), kinetic parameters, viability and acrosomal integrity. Pregnancy rate after insemination was similar for samples collected using AV, and the G0, G6, G12 and G24 samples. In conclusion, ovine epididymides can be exposed to room temperature, for up to 24 h post mortem, with no effect on viability and fertility of cryopreserved seminal samples. The addition of seminal plasma to the cryopreservation extender had no effect on spermatozoa quality nor fertility. Department of Technology Postgraduate Studies in Bioprocess Engineering and Biotechnology Human and Animal Health Federal University of Paraná, Rua dos Funcionários, 1540 Department of Veterinary Medicine Postgraduate Studies in Animal Science Pontifical Catholic University of Paraná, Rua Imaculada Conceição, 1155, Prado Velho Department of Animal Reproduction and Veterinary Radiology- FMVZ – UNESP, Distrito de Rubião Júnior, S/n Department of Animal Reproduction and Veterinary Radiology- FMVZ – UNESP, Distrito de Rubião Júnior, S/n
Databáze: OpenAIRE
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