Neuroprotective effects of topical coenzyme Q10 + vitamin E in mechanic optic nerve injury model

Autor: Selcan Ekicier Acar, Aysel Çolak, M Sinan Sarıcaoğlu, Zeynep Aktas, Aylin Sepici Dinçel
Rok vydání: 2019
Předmět:
Male
Retinal Ganglion Cells
0301 basic medicine
Nervous system
Ubiquinone
medicine.medical_treatment
Blotting
Western
bcl-X Protein
Glaucoma
Administration
Ophthalmic
Pharmacology
Neuroprotection
Cofactor
03 medical and health sciences
0302 clinical medicine
Optic nerve injury
Glial Fibrillary Acidic Protein
medicine
Animals
Vitamin E
Rats
Wistar
bcl-2-Associated X Protein
Transcription Factor Brn-3A
Retina
biology
business.industry
Calcium-Binding Proteins
Microfilament Proteins
General Medicine
medicine.disease
Immunohistochemistry
Rats
Disease Models
Animal
Drug Combinations
Ophthalmology
Neuroprotective Agents
030104 developmental biology
medicine.anatomical_structure
Retinal ganglion cell
Optic Nerve Injuries
biology.protein
Ophthalmic Solutions
business
030217 neurology & neurosurgery
Zdroj: European Journal of Ophthalmology. 30:714-722
ISSN: 1724-6016
1120-6721
Popis: Purpose: We aimed to create mechanic optic nerve injury model in rats and investigate the neuroprotective effects of topical Coenzyme Q10 + Vitamin E (CoQ + Vit.E) molecules on retinal ganglion cells. Methods: Mechanic optic nerve injury model was created in the right eyes of rats (n = 12). Rats were divided into two groups: glaucoma model with sham treatment and topical CoQ + Vit.E treatment. Treatment was applied for 4 weeks. Glial fibrillary acidic protein, Brn-3a antibody, and anti-Iba1 were examined by immunohistochemistry. Glial fibrillary acidic protein, Bax, Bcl-xL, and Tfam protein expression were measured by Western blot analysis. Results: The number of Brn-3a-positive retinal ganglion cell was 15.0 ± 1.0 (min: 14, max: 16) in sham treatment group and 22.2 ± 4.8 (min: 18, max: 29) in topical CoQ10 + Vit.E treatment group. The protection of Brn-3a in CoQ10 + Vit.E was statistically significant (p Conclusion: This study has shown that in glaucoma treatment the neuron protecting effect of topical CoQ10 + Vit.E molecules can be valuable.
Databáze: OpenAIRE
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