Multicenter evaluation of a 1-h enzyme-linked immunosorbent assay for rubella serology
| Autor: | E Buimovici-Klein, K J Barnes, A J O'Beirne, W L Boteler |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Microbiology (medical)
Hemagglutination Inhibition Tests Population Enzyme-Linked Immunosorbent Assay Biology medicine.disease_cause Antibodies Viral Rubella Serology Immunoenzyme Techniques medicine Humans Seroconversion education education.field_of_study Hemagglutination assay Rubella virus medicine.disease Virology Molecular biology Titer Immunoglobulin M Evaluation Studies as Topic Immunoglobulin G Research Article |
| Zdroj: | Journal of clinical microbiology. 20(6) |
| ISSN: | 0095-1137 |
| Popis: | A 1-h enzyme-linked immunosorbent assay (Rubestat) was developed for rubella virus immunoglobulin G detection. The assay used phenolphthalein monophosphate as the substrate, which, when developed, can easily be read visually. Rubestat compared very favorably to hemagglutination inhibition and commercial enzyme-linked immunosorbent assays in its ability to determine immune status. Rubestat demonstrated greater than 97% specificity, sensitivity, and accuracy as compared with other methodologies at 10 different laboratories. The Rubestat index values were precise, with coefficients of variation for intra- and interassay variation of less than 10%. Mean index values had a linear correlation with hemagglutination inhibition titers (r2 greater than 0.97). A population distribution of index values illustrated two distinct bell-shaped curves representing the positive and negative populations. Studies of acute and convalescent serum pairs showed Rubestat to be as accurate as hemagglutination inhibition in determining seroconversion. |
| Databáze: | OpenAIRE |
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