Dextran Sulfate/Pramlintide Polyelectrolyte Nanoparticles as a Promising Delivery System: Optimization, Evaluation of Supramolecular Interactions and Effect on Conformational Stability of the Peptide Drug
| Autor: | Elenara Lemos-Senna, Vanessa A. de Oliveira, Francisco H. Xavier Jr., Carine Zuglianello, Andrés F. Chamorro |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Isothermal microcalorimetry
Circular dichroism Chemistry polyelectrolyte nanoparticles technology industry and agriculture Supramolecular chemistry Nanoparticle pramlintide General Chemistry supramolecular interactions Polyelectrolyte dextran sulfate Dynamic light scattering Chemical engineering Zeta potential peptide drug delivery Nanogel |
| Zdroj: | Journal of the Brazilian Chemical Society v.32 n.8 2021 Journal of the Brazilian Chemical Society Sociedade Brasileira de Química (SBQ) instacron:SBQ Journal of the Brazilian Chemical Society, Volume: 32, Issue: 8, Pages: 1642-1653, Published: 28 JUL 2021 |
| ISSN: | 0103-5053 |
| Popis: | In this study, we investigated the feasibility to obtain nanoparticles (NPs) by assembling pramlintide (Pram) with dextran sulfate (DexS), as a new approach for mucosal peptide delivery. DexS/Pram NPs were prepared by dropwise addition of a Pram solution to a DexS solution under magnetic stirring. The physicochemical characteristics of NPs and molecular interactions involved in the co-assembling were evaluated by dynamic light scattering (DLS), transmission electronic microscopy (TEM), isothermal titration microcalorimetry, Fourier-transform infrared spectroscopy (FTIR), fluorescence quenching, and circular dichroism (CD). DexS/Pram NPs displayed a narrow size distribution (ca. 200 nm), negative zeta potential (ca. −40 mV), association efficiency close to 100%, and nanogel behavior. The assembling with DexS increased the Pram α-helical content, stabilizing the peptide in its bioactive form. The colloidal stability of nanoparticles was dependent on the salt concentration and it could be assumed that peptide release from nanoparticles occurs by dissociation of the complex at physiological conditions. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|