Making, Testing, and Using Potassium Ion Selective Microelectrodes in Tissue Slices of Adult Brain
| Autor: | J. Christopher Octeau, Guido C. Faas, Istvan Mody, Baljit S. Khakh |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
1.1 Normal biological development and functioning General Chemical Engineering K+ Hippocampus General Biochemistry Genetics and Molecular Biology neuroscience Mice 03 medical and health sciences astrocyte Slice preparation Issue 135 Underpinning research homeostasis medicine Extracellular Animals Psychology mouse Ion channel Action potential initiation Membrane potential General Immunology and Microbiology Chemistry General Neuroscience Neurosciences Brain electrophysiology Electric Stimulation brain slice Electrophysiology ion-selective microelectrode 030104 developmental biology medicine.anatomical_structure Neurological Potassium Biophysics synapses Cognitive Sciences Biochemistry and Cell Biology Microelectrodes Homeostasis Neuroscience Astrocyte |
| Zdroj: | Journal of Visualized Experiments. |
| ISSN: | 1940-087X |
| Popis: | Potassium ions significantly contribute to the resting membrane potential of cells and, therefore, extracellular K(+) concentration is a crucial regulator of cell excitability. Altered concentrations of extracellular K(+) affect the resting membrane potential and cellular excitability by shifting the equilibria between closed, open and inactivated states for voltage-dependent ion channels that underlie action potential initiation and conduction. Hence, it is valuable to directly measure extracellular K(+) dynamics in health and diseased states. Here, we describe how to make, calibrate and use monopolar K(+)-selective microelectrodes. We deployed them in adult hippocampal brain slices to measure electrically evoked K(+) concentration dynamics. The judicious use of such electrodes is an important part of the tool-kit needed to evaluate cellular and biophysical mechanisms that control extracellular K(+) concentrations in the nervous system. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|