Genomic diversity and organization of complex polysaccharide biosynthesis clusters in the genus Dickeya

Autor: Suchi Srivastava, Devanshi Khokhani, Manish Ranjan, Ashish Ranjan, Sanjeeva Nayaka, Zachary P. Keyser
Rok vydání: 2020
Předmět:
Glycobiology
Sequence Homology
Plant Science
Toxicology
Pathology and Laboratory Medicine
Biochemistry
Homology (biology)
Medicine and Health Sciences
Coding region
Toxins
Phylogeny
Genetics
0303 health sciences
Multidisciplinary
biology
Bacterial Genomics
Plant Bacterial Pathogens
Microbial Genetics
O Antigens
Genomics
Multigene Family
Horizontal gene transfer
Medicine
Cell envelope
Research Article
Gene Transfer
Horizontal
Science
Toxic Agents
Bacterial Toxins
Plant Pathogens
Virulence
Dickeya
Microbial Genomics
Biosynthesis
Microbiology
03 medical and health sciences
Open Reading Frames
Phylogenetics
Polysaccharides
Bacterial Genetics
Gene Prediction
Gene
030304 developmental biology
Plant Diseases
Base Sequence
030306 microbiology
Genetic Variation
Biology and Life Sciences
Computational Biology
Bacteriology
Plant Pathology
biology.organism_classification
Genome Analysis
Endotoxins
Genetic Loci
Genome
Bacterial
Zdroj: PLoS ONE
PLoS ONE, Vol 16, Iss 2, p e0245727 (2021)
ISSN: 1932-6203
Popis: The pectinolytic genus Dickeya (formerly Erwinia chrysanthemi) comprises numerous pathogenic species which cause diseases in various crops and ornamental plants across the globe. Their pathogenicity is governed by complex multi-factorial processes of adaptive virulence gene regulation. Extracellular polysaccharides and lipopolysaccharides present on bacterial envelope surface play a significant role in the virulence of phytopathogenic bacteria. However, very little is known about the genomic location, diversity, and organization of the polysaccharide and lipopolysaccharide biosynthetic gene clusters in Dickeya. In the present study, we report the diversity and structural organization of the group 4 capsule (G4C)/O-antigen capsule, putative O-antigen lipopolysaccharide, enterobacterial common antigen, and core lipopolysaccharide biosynthesis clusters from 54 Dickeya strains. The presence of these clusters suggests that Dickeya has both capsule and lipopolysaccharide carrying O-antigen to their external surface. These gene clusters are key regulatory components in the composition and structure of the outer surface of Dickeya. The O-antigen capsule/group 4 capsule (G4C) coding region shows a variation in gene content and organization. Based on nucleotide sequence homology in these Dickeya strains, two distinct groups, G4C group I and G4C group II, exist. However, comparatively less variation is observed in the putative O-antigen lipopolysaccharide cluster in Dickeya spp. except for in Dickeya zeae. Also, enterobacterial common antigen and core lipopolysaccharide biosynthesis clusters are present mostly as conserved genomic regions. The variation in the O-antigen capsule and putative O-antigen lipopolysaccharide coding region in relation to their phylogeny suggests a role of multiple horizontal gene transfer (HGT) events. These multiple HGT processes might have been manifested into the current heterogeneity of O-antigen capsules and O-antigen lipopolysaccharides in Dickeya strains during its evolution.
Databáze: OpenAIRE
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