Regulation of laccase synthesis in induced Neurospora crassa cultures
| Autor: | U A Germann, Konrad Lerch, B C Schilling, R M Linden |
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| Rok vydání: | 1991 |
| Předmět: |
Blotting
Western Biology Cycloheximide Gene Expression Regulation Enzymologic Neurospora crassa chemistry.chemical_compound Gene Expression Regulation Fungal Gene expression Genetics Extracellular RNA Messenger Regulation of gene expression Laccase Fungal genetics RNA Fungal General Medicine biology.organism_classification De novo synthesis Kinetics chemistry Biochemistry Enzyme Induction Oxidoreductases |
| Zdroj: | Current Genetics. 19:375-381 |
| ISSN: | 1432-0983 0172-8083 |
| Popis: | Rapidly growing cultures of N. crassa do not produce laccase. Exposure of this fungus to different inducing agents leads to a de novo biosynthesis of extracellular laccase in vegetative cultures. In this study the induction of laccase after addition of cycloheximide and D-phenylalanine is reported. De novo synthesis of laccase mRNA was followed over 96 h after induction. A fast appearance of the message, as well as its presence over a rather long period, indicates a regulation on a transcriptional and maybe on a post-transcriptional level. In contrast to the kinetics of mRNA production, Western analysis with a polyclonal anti-laccase antibody showed a remarkably delayed appearance of the intracellular, as well as of the extracellular, protein product after induction with cycloheximide. Furthermore, activity measurements at different times after induction of both crude extracts and media of the vegetative cultures showed that in extracted mycelia the activity occurs at least 20 h after the protein is immunologically detectable. Laccase activity in the medium starts to increase only 30 h after translation. These data, together with the published structure of the laccase gene, indicate a regulation on the transcriptional, post-transcriptional and on a post-translational level. In cultures induced with D-phenylalanine a rather fast appearance of laccase-specific mRNA also indicates a transcriptional regulation. Compared to cycloheximide-induced laccase biosynthesis no delayed appearance of laccase protein levels of laccase activity is observed after induction with D-phenylalanine. |
| Databáze: | OpenAIRE |
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