Noncanonical contributions of MutLγ to VDE-initiated crossovers during Saccharomyces cerevisiae meiosis
| Autor: | Darpan Medhi, Anura Shodhan, Michael Lichten |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Saccharomyces cerevisiae
MLH3 QH426-470 Biology PCH2 03 medical and health sciences 0302 clinical medicine Meiosis Centromere Genetics meiosis budding yeast URA3 Molecular Biology Genetics (clinical) 030304 developmental biology 0303 health sciences Chemistry fungi Chromosome biology.organism_classification recombination Homologous recombination 030217 neurology & neurosurgery Recombination |
| Zdroj: | G3: Genes, Genomes, Genetics, Vol 9, Iss 5, Pp 1647-1654 (2019) |
| DOI: | 10.1101/510297 |
| Popis: | InSaccharomyces cerevisiae, the meiosis-specific axis proteins Hop1 and Red1 are present nonuniformly across the genome. In a previous study, the meiosis-specificVMAl-derivedendonuclease (VDE) was used to examine Spo11-independent recombination in a recombination reporter inserted in a Hop1/Red1-enriched region (HIS4) and in a Hop1/Red1-poor region (URA3). VDE-initiated crossovers atHIS4were mostly dependent on Mlh3, a component of the MutLγ meiotic recombination intermediate resolvase, while VDE-initiated crossovers atURA3were mostly Mlh3-independent. These differences were abolished in the absence of the chromosome axis remodeler Pch2, and crossovers at both loci become partly Mlh3-dependent. To test the generality of these observations, we examined inserts at six additional loci that differed in terms of Hop1/Red1 enrichment, chromosome size, and distance from centromeres and telomeres. All six loci behaved similarly toURA3: the vast majority of VDE-initiated crossovers were Mlh3-independent. This indicates that, counter to previous suggestions, meiotic chromosome axis protein enrichment is not a primary determinant of which recombination pathway gives rise to crossovers during VDE-initiated meiotic recombination. Inpch2Δ mutants, the fraction of VDE-induced crossovers that were Mlh3-dependent increased to levels previously observed for Spo11-initiated crossovers inpch2Δ, indicating that Pch2-dependent processes play an important role in controlling the distribution of factors necessary for MutLγ-dependent crossovers. |
| Databáze: | OpenAIRE |
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