NLRP3 receptor contributes to protection against experimental antigen-mediated cholangitis
| Autor: | José L. Reyes, Bertus Eksteen, Danielle T. Vannan, Marisol I. González |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Male Chemokine Immunology & Inflammation Cholangitis Ovalbumin Biophysics Context (language use) Inflammation Cell Death & Injury Biochemistry Severity of Illness Index Liver inflammation 03 medical and health sciences 0302 clinical medicine Immune system Antigen NLRP3 Fibrosis NLR Family Pyrin Domain-Containing 3 Protein medicine Animals Obesity Antigens Receptor Molecular Biology Research Articles Mice Knockout biology integumentary system business.industry Interleukin-6 Cell Biology medicine.disease Gastrointestinal Renal & Hepatic Systems Chemokine CXCL10 Mice Inbred C57BL Disease Models Animal 030104 developmental biology Bile Ducts Intrahepatic 030220 oncology & carcinogenesis Immunology biology.protein Tumor necrosis factor alpha medicine.symptom Inflammation Mediators business Signal Transduction |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | Inflammatory diseases of the bile ducts like primary sclerosing colangitis (PSC) are characterized by a robust cellular response targeting the biliary epithelium leading to chronic inflammation and fibrosis. Driving fibro-inflammatory diseases, NOD-like receptors such as NLRP3 have been identified as a central component to immune-mediated pathology. However, to date the role of NLRP3 in biliary diseases has been poorly explored. Here, we addressed the role of NLRP3 in the OVAbil mouse model of antigen-mediated cholangitis. As obesity continues to spread worldwide, we also evaluated the NLRP3 response in experimental cholangitis after high-fat diet exposure. We compared the extent of histopathological liver damage between OVAbil and OVAbilxNLRP3−/− mice after either a standard chow or a high-fat diet. Infiltrating immune cells were characterized by flow cytometry and levels of cytokines, chemokines and liver enzymes in blood samples were analyzed at the end of the experiment. We observed a more severe histopathological phenotype of cholangitis in absence of NLRP3, characterized by loss of bile ducts and larger inflammatory foci and higher levels of IL- 6 and CXCL10 as compared with NLRP3 sufficient mice. This phenotype was further exaggerated in the context of obesity, where cholangitis induced in NLRP3-deficient obese mice resulted in further exacerbated histopathology and increased levels of IL-13 and TNFα, suggesting a diet-specific profile. The absence of NLRP3 caused a supressed IL-17 response. In summary, our data suggest that activation of NLRP3 attenuates this antigen-mediated OVAbil model of cholangitis. |
| Databáze: | OpenAIRE |
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