A facile system for construction of HSV-1 variants: site directed mutation of the UL26 protease gene in HSV-1
| Autor: | Register Rb, Shafer Ja |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Genes
Viral viruses medicine.medical_treatment Mutant Molecular Sequence Data Mutagenesis (molecular biology technique) Herpesvirus 1 Human Biology medicine.disease_cause Virus Viral Proteins Virology Chlorocebus aethiops medicine Animals Humans Amino Acid Sequence Site-directed mutagenesis Vero Cells Cell Line Transformed Mutation Protease Binding Sites Base Sequence Point mutation Serine Endopeptidases Molecular biology NS2-3 protease DNA Viral Mutagenesis Site-Directed Rabbits |
| Zdroj: | Journal of virological methods. 57(2) |
| ISSN: | 0166-0934 |
| Popis: | A 2-plasmid/4-cosmid-based system of mutagenesis is described for construction of herpes simplex virus type 1 (HSV-1) variants with point mutations in the protease gene. The system was used to reconstruct a mutant virus (V701) with Tyr30 to Phe and Ala48 to Val mutations in HSV-1 protease that exhibits the temperature sensitive phenotype of the previously characterized temperature sensitive HSV-1 mutant, ts1201. The 2-plasmid/4-cosmid system of mutagenesis was further validated by using it to construct a virus wherein the active site Ser129 of HSV-1 protease was mutated to Ala. The resulting virus mutant (V713) grew only on the Vero host range cell line PHS-23. In V713 infected Vero cells, the processing of Pra to No was almost completely blocked, and B capsids accumulated in the nucleus in crystal-like aggregates, suggesting that protease activity is required for emergence of monodispersed capsids from these aggregates. Back mutation of Ala129 to Ser using the V713 viral DNA as template for PCR mutagenesis restored the wild-type phenotype verifying that the replicative incompetence of V713 reflected only the effect of the Ser to Ala mutation. The 2-plasmid/4-cosmid system of mutagenesis (and modifications thereof) should facilitate production of new mutant viruses for delineating interactions of domains of HSV-1 protease (as well as other HSV-1 proteins) important for virus replication. |
| Databáze: | OpenAIRE |
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