Novel Alginate-Chitosan Composite Microspheres for Implant Delivery of Vancomycin andIn VivoEvaluation
| Autor: | Yongbiao Ge, Ming Zhao, Yimin Mao, Jiang Fan |
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| Rok vydání: | 2016 |
| Předmět: |
Male
0301 basic medicine Alginates 030106 microbiology 02 engineering and technology Biochemistry Chitosan 03 medical and health sciences chemistry.chemical_compound Glucuronic Acid Pharmacokinetics Vancomycin In vivo Drug Discovery medicine Animals Chromatography High Pressure Liquid Drug Implants Pharmacology Hexuronic Acids Organic Chemistry 021001 nanoscience & nanotechnology Glucuronic acid Microspheres Anti-Bacterial Agents chemistry Emulsion Microscopy Electron Scanning Molecular Medicine Female Rabbits Implant Particle size 0210 nano-technology Biomedical engineering medicine.drug |
| Zdroj: | Chemical Biology & Drug Design. 88:434-440 |
| ISSN: | 1747-0277 |
| Popis: | In this study, vancomycin loaded alginate-chitosan composite microspheres were developed by emulsion cross-linking method. The in vitro and vivo characterizations were done to evaluate the feasibility of application. Our experimental results showed that the emulsification cross-linking technique appeared to be a feasible method for the preparation of alginate-chitosan composite microspheres. The microspheres were spherical in shape and the mean particle size and drug loading were 25.3 ± 5.4 μm and 18.5 ± 2.3% respectively. A sustained vancomycin release was realized i.e. the amount of cumulative release increased in a time frame of 24 h to reach an amount i.e. ~68%. The model that fit best for vancomycin released from the microspheres was the Higuchi kinetic model with a correlation coefficient r = 0.9996. In vivo results showed that the application of microspheres not only reduced the toxicity, but also maintained effective drug concentration. In addition, no severe signs of epithelial necrosis and sloughing of epithelial cells were detected in histological studies. |
| Databáze: | OpenAIRE |
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