HLA-G Expression in Human Embryonic Stem Cells and Preimplantation Embryos
| Autor: | Ileana Mateizel, Marc De Waele, Martine Vercammen, Greet Cauffman, Joel LeMaoult, Edgardo D. Carosella, Peter A. Horn, Paul Devroey, A. Verloes, Hilde Van De Velde, Vera Rebmann |
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| Přispěvatelé: | Hematology, Department of Embryology and Genetics, Reproduction and Genetics, Immunology and Microbiology |
| Rok vydání: | 2011 |
| Předmět: |
Cleavage Stage
Ovum HLA-G Immunology Medizin Stem cells Biology Cell therapy Leukocyte Immunoglobulin-like Receptor B1 Antigens CD HLA Antigens Cell Line Tumor Immune Tolerance medicine Humans Protein Isoforms Immunology and Allergy Inner cell mass Blastocyst Receptors Immunologic Cells Cultured Embryonic Stem Cells reproductive and urinary physiology HLA-G Antigens Histocompatibility Antigens Class I human embryonic stem cells Acquired immune system Embryonic stem cell Cell biology Transplantation medicine.anatomical_structure Gene Expression Regulation IVF Blastocyst Inner Cell Mass embryonic structures Cancer cell Oocytes Stem cell Protein Binding |
| Zdroj: | The Journal of Immunology. 186:2663-2671 |
| ISSN: | 1550-6606 0022-1767 |
| Popis: | Human leukocyte Ag-G, a tolerogenic molecule that acts on cells of both innate and adaptive immunity, plays an important role in tumor progression, transplantation, placentation, as well as the protection of the allogeneic fetus from the maternal immune system. We investigated HLA-G mRNA and protein expression in human embryonic stem cells (hESC) derived from the inner cell mass (ICM) of blastocysts. hESC self-renew indefinitely in culture while maintaining pluripotency, providing an unlimited source of cells for therapy. HLA-G mRNA was present in early and late passage hESC, as assessed by real time RT-PCR. Protein expression was demonstrated by flow cytometry, immunocytochemistry, and ELISA on an hESC extract. Binding of HLA-G with its ILT2 receptor demonstrated the functional active status. To verify this finding in a physiologically relevant setting, HLA-G protein expression was investigated during preimplantation development. We demonstrated HLA-G protein expression in oocytes, cleavage stage embryos, and blastocysts, where we find it in trophectoderms but also in ICM cells. During blastocyst development, a downregulation of HLA-G in the ICM cells was present. This data might be important for cell therapy and transplantation because undifferentiated hESC can contaminate the transplant of differentiated stem cells and develop into malignant cancer cells. |
| Databáze: | OpenAIRE |
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