Simplifying the Proteome: Analytical Strategies for Improving Peak Capacity

Autor:	Lee A. Gethings, Joanne B. Connolly
Rok vydání:	2014
Předmět:	Analyte Identification (information) Proteomics methods Workflow Computer science Dynamic range Proteome Biochemical engineering Limiting Mass spectrometry
Zdroj:	Advances in Experimental Medicine and Biology ISBN: 9783319060675 Advances in Experimental Medicine and Biology ISBN: 9783030159498
DOI:	10.1007/978-3-319-06068-2_3
Popis:	The diversity of biological samples and dynamic range of analytes being analyzed can prove to be an analytical challenge and is particularly prevalent to proteomic studies. Maximizing the peak capacity of the workflow employed can extend the dynamic range and increase identification rates. The focus of this chapter is to present means of achieving this for various analytical techniques such as liquid chromatography, mass spectrometry, and ion mobility. A combination of these methods can be used as part of a data-independent acquisition strategy, thereby limiting issues such as chimericy when analyzing regions of extreme analyte density.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::36cf35fde678235fe20fb4bcb825e763 https://doi.org/10.1007/978-3-319-06068-2_3 Zobrazit plný text záznamu