Silanization of Chitosan and Hydrogel Preparation for Skeletal Tissue Engineering
| Autor: | Fabienne Jordana, Cécile Boyer, Pierre Weiss, Amadou Toure, Julie Lesoeur, Jérôme Guicheux, Kouakou Luc Kouadio, Boris Halgand, Gildas Rethore |
|---|---|
| Přispěvatelé: | Regenerative Medicine and Skeleton research lab (RMeS), Ecole Nationale Vétérinaire, Agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Nantes - UFR Odontologie, Université de Nantes (UN), Ostéo-articulaire - Tête et cou - Odontologie - Neurochirurgie - Neurotraumatologie [CHU Nantes] (Pôle hospitalo-universitaire PHU4 - OTONN), Centre hospitalier universitaire de Nantes (CHU Nantes), Université Cheikh Anta Diop [Dakar, Sénégal] (UCAD), Regenerative Medicine and Skeleton (RMeS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre hospitalier universitaire de Nantes (CHU Nantes)-Université de Nantes - UFR de Médecine et des Techniques Médicales (UFR MEDECINE), Université de Nantes (UN)-Université de Nantes (UN)-École nationale vétérinaire, agroalimentaire et de l'alimentation Nantes-Atlantique (ONIRIS), Jehan, Frederic |
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
[SDV.MHEP.AHA] Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO]
Polymers and Plastics silane 02 engineering and technology macromolecular substances Article Chitosan Extracellular matrix lcsh:QD241-441 03 medical and health sciences chemistry.chemical_compound Tissue engineering lcsh:Organic chemistry [SDV.MHEP.AHA]Life Sciences [q-bio]/Human health and pathology/Tissues and Organs [q-bio.TO] Viability assay cartilage 030304 developmental biology 0303 health sciences [SDV.MHEP.RSOA] Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system [SDV.MHEP.GEG] Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology Chemistry [SDV.MHEP.GEG]Life Sciences [q-bio]/Human health and pathology/Geriatry and gerontology technology industry and agriculture Biomaterial General Chemistry 021001 nanoscience & nanotechnology Transplantation [SDV.MHEP.RSOA]Life Sciences [q-bio]/Human health and pathology/Rhumatology and musculoskeletal system Silanization tissue engineering Self-healing hydrogels Biophysics chitosan hydrogel 0210 nano-technology |
| Zdroj: | Polymers Polymers, MDPI, 2020, 12 (12), pp.2823. ⟨10.3390/polym12122823⟩ Volume 12 Issue 12 Polymers, Vol 12, Iss 2823, p 2823 (2020) |
| ISSN: | 2073-4360 |
| DOI: | 10.3390/polym12122823⟩ |
| Popis: | International audience; Tissue engineering is a multidisciplinary field that relies on the development of customized biomaterial to support cell growth, differentiation and matrix production. Toward that goal, we designed the grafting of silane groups onto the chitosan backbone (Si-chito) for the preparation of in situ setting hydrogels in association with silanized hydroxypropyl methylcellulose (Si-HPMC). Once functionalized, the chitosan was characterized, and the presence of silane groups and its ability to gel were demonstrated by rheology that strongly suggests the presence of silane groups. Throughout physicochemical investigations, the Si-HPMC hydrogels containing Si-chito were found to be stiffer with an injection force unmodified. The presence of chitosan within the hydrogel has demonstrated a higher adhesion of the hydrogel onto the surface of tissues. The results of cell viability assays indicated that there was no cytotoxicity of Si-chito hydrogels in 2D and 3D culture of human SW1353 cells and human adipose stromal cells, respectively. Moreover, Si-chito allows the transplantation of human nasal chondrocytes in the subcutis of nude mice while maintaining their viability and extracellular matrix secretory activity. To conclude, Si-chito mixed with Si-HPMC is an injectable, self-setting and cytocompatible hydrogel able to support the in vitro and in vivo viability and activity of hASC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|