Connexin-mediated communication controls cell proliferation and is essential in retinal histogenesis
| Autor: | Luiz R.G. Britto, Taisa de Oliveira Santos, Rodrigo R. Resende, Priscilla Sayami Akamine, Leandro M. Castro, Edgard J. Osuna-Melo, Alexandre Hiroaki Kihara, Kallene Summer Moreira Vidal, Dânia Emi Hamassaki, Vera Paschon |
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| Rok vydání: | 2009 |
| Předmět: |
Time Factors
Connexin Cell Communication Histogenesis Biology Retinal Horizontal Cells Connexins Retina chemistry.chemical_compound Developmental Neuroscience Proliferating Cell Nuclear Antigen Neural Pathways medicine Animals Progenitor cell Rats Wistar Cell Proliferation Quinine Stem Cells Gene Expression Regulation Developmental Retinal Cell cycle Cell biology Rats medicine.anatomical_structure chemistry Carbenoxolone Neuroglia sense organs Stem cell Developmental Biology Central Nervous System Agents |
| Zdroj: | International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience. 28(1) |
| ISSN: | 1873-474X |
| Popis: | Connexin (Cx) channels and hemichannels are involved in essential processes during nervous system development such as apoptosis, propagation of spontaneous activity and interkinetic nuclear movement. In the first part of this study, we extensively characterized Cx gene and protein expression during retinal histogenesis. We observed distinct spatio-temporal patterns among studied Cx and an overriding, ubiquitous presence of Cx45 in progenitor cells. The role of Cx-mediated communication was assessed by using broad-spectrum (carbenoxolone, CBX) and Cx36/Cx50 channel-specific (quinine) blockers. In vivo application of CBX, but not quinine, caused remarkable reduction in retinal thickness, suggesting changes in cell proliferation/apoptosis ratio. Indeed, we observed a decreased number of mitotic cells in CBX-injected retinas, with no significant changes in the expression of PCNA, a marker for cells in proliferative state. Taken together, our results pointed a pivotal role of Cx45 in the developing retina. Moreover, this study revealed that Cx-mediated communication is essential in retinal histogenesis, particularly in the control of cell proliferation. |
| Databáze: | OpenAIRE |
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